Table 2 Primers
Name | Sequence 5' to 3' | Usage |
|---|---|---|
attB1 | ggggacaagtttgtacaaaaaagcaggct | Sequencing of coding sequence after ORFeome screening |
attB2 | ggggaccactttgtacaagaaagctgggt | Sequencing of coding sequence after ORFeome screening |
acoDHP1 | tagcaaatgcagtgcaag | PCR amplification for checking aidB disruption |
pGEM-T-aval | ggaaacagctatgacca | PCR amplification for checking aidB disruption |
AcoA | gcggcttacgggccataaa | Amplification of B. abortus aidB internal fragment |
AcoB | gctgctcgaccaaaggcttg | Amplification of B. abortus aidB internal fragment |