Table 1 Strains and plasmids
Strain | Relevant Genotype or Description | Reference or Source |
|---|---|---|
B. abortus | Â | Â |
544 NalR | Nalidixic acid-resistant B. abortus 544 | J-M. Verger |
XDB1155 | B. abortus 544 pdhS-cfp | [16] |
XDB1120 | XDB1155 + pDD001 | This study |
XDB1121 | Disrupted aidB in B. abortus 544 NalR | This study |
XDB1122 | XDB1155 + pDD003 | This study |
XDB1123 | XDB1155 + pDD007 | This study |
XDB1124 | XDB1155 + pDD008 | This study |
XDB1127 | XDB1121 + pDD001 | This study |
XDB1118 | B. abortus 544 with integrated pCVDH07 | This study and [33] |
XDB1128 | XDB1118 + pDD001 | This study |
E. coli | Â | Â |
DH10B | Cloning strain | Invitrogen |
S17-1 | RP4-2, Tc::Mu,Km-Tn7, for plasmid mobilization | [26] |
Plasmid | Relevant Genotype or Description | Reference or Source |
pDONR201 | BP cloning vector | Invitrogen |
pRH005 | Gateway-compatible YFP low copy vector | [34] |
pRH016 | Gateway-compatible pBBR1-MCS1-3HA | [34] |
pDD001 | pRH005 carrying aidB | This study |
pDD002 | pDONR201 carrying aidB | This study |
pDD003 | pRH016 carrying aidB | This study |
pDD007 | pRH016 carrying acaD1 | This study |
pDD008 | pRH016 carrying acaD2 | This study |