|
B. abortus
| | |
|
544 NalR
|
Nalidixic acid-resistant B. abortus 544
|
J-M. Verger
|
|
XDB1155
|
B. abortus 544 pdhS-cfp
|
[16]
|
|
XDB1120
|
XDB1155 + pDD001
|
This study
|
|
XDB1121
|
Disrupted aidB in B. abortus 544 NalR
|
This study
|
|
XDB1122
|
XDB1155 + pDD003
|
This study
|
|
XDB1123
|
XDB1155 + pDD007
|
This study
|
|
XDB1124
|
XDB1155 + pDD008
|
This study
|
|
XDB1127
|
XDB1121 + pDD001
|
This study
|
|
XDB1118
|
B. abortus 544 with integrated pCVDH07
|
This study and [33]
|
|
XDB1128
|
XDB1118 + pDD001
|
This study
|
|
E. coli
| | |
|
DH10B
|
Cloning strain
|
Invitrogen
|
|
S17-1
|
RP4-2, Tc::Mu,Km-Tn7, for plasmid mobilization
|
[26]
|
|
Plasmid
|
Relevant Genotype or Description
|
Reference or Source
|
|
pDONR201
|
BP cloning vector
|
Invitrogen
|
|
pRH005
|
Gateway-compatible YFP low copy vector
|
[34]
|
|
pRH016
|
Gateway-compatible pBBR1-MCS1-3HA
|
[34]
|
|
pDD001
|
pRH005 carrying aidB
|
This study
|
|
pDD002
|
pDONR201 carrying aidB
|
This study
|
|
pDD003
|
pRH016 carrying aidB
|
This study
|
|
pDD007
|
pRH016 carrying acaD1
|
This study
|
|
pDD008
|
pRH016 carrying acaD2
|
This study
|
