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Table 1 List of strains and plasmids

From: Integration of a laterally acquired gene into a cell network important for growth in a strain of Vibrio rotiferianus

Strain or plasmid Relevant genotypea Reference or source
Strains   
V. rotiferianus DAT722   
DAT722 Wild-type [11]
DAT722-Sm DAT722; Spontaneous SmR mutant. This study
MD7 DAT722-Sm; Single recombination cross-over of pVSD2 into cassette 61, KmR This study
d8-60a DAT722-Sm; Δcassettes 8-60, SmR, KmR This study
d8-60b DAT722-Sm; Δcassettes 8-60, SmR, KmR This study
d8-60b-S DAT722-Sm; Δcassettes 8-60, SmR, KmR. Spontaneous mutant of d8-60b. This study
d8-60c DAT722-Sm; Δcassettes 8-60, SmR, KmR This study
d8-60c-S DAT722-Sm; Δcassettes 8-60, SmR, KmR. Spontaneous mutant of d8-60c. This study
d16-60 DAT722-Sm; Δcassettes 16-60, SmR, KmR This study
E. coli   
XL1-Blue F' proAB lacIqZΔM15 Tn10/recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relAi, TcR Stratagene
SY327 λ pir Δ(lac pro) argE (Am) rif nalA recA56 [38]
SM10 λ pir thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu, Tcr KmR [39]
Plasmids   
pLOW2 Cloning vector, KmR [40]
pGEM-T Easy Cloning vector, ApR Promega
pMAQ1080 pGEM-T Easy carrying a 1834-bp fragment. The fragment was created using fusion PCR and consists of, in order, a 448-bp of paralog group 1 sequence, a 964-bp fragment containing aphA1 and a 410-bp paralog group 2 sequence abutted by salI restriction sites. This study
pCVD442 Mobilisable sacB counter-selectable suicide vector, ApR [41]
RK600 pJAK16 pMAQ1081 pMAQ1082 ColE1 oriV; RP4tra+ RP4 oriT; CmR; helper plasmid in triparental matings Low copy IPTG-inducible expression vector, CmR salI fragment from pMAQ1080 cloned into the unique salI site of pCVD442. pJAK16 containing cassette 11 [42] [43] This study This study
  1. aSmR, streptomycin resistance; KmR, kanamycin resistance; TcR, tetracycline resistance; ApR, Ampicillin resistance