Table 5 Primers designed for the expression of VP1s and VP4s
primers | sequences | endonuclease | fragments (bp) |
|---|---|---|---|
EV71-VP1-2F | 5'- ACGGATCCGGAGATAGGGTGGCAGAT -3' | BamH| | Â |
EV71-VP1-2R | 5'- CCTCTCGAGTTAAAGAGTGGTGATCGCTGTG -3' | Xho| | 911 |
EV71-VP4-2F | 5'-CCGAATTCATGGGCTCGCAAGTG-3' | EcoR| | Â |
EV71VP4-2R | 5'-TCTCTCGAGTTACTTCAGCGGCGCTGC-3' | Xho| | 227 |
CA16-VP1-2F | 5'- GAAGATCTGGGGGATCCTATCGCAGAC-3' | BamH| | Â |
CA16-VP1-2R | 5'- CCGCTCGAGTTACAGTGTTGTTATCTTG-3' | Xho| | 912 |
CA16-VP4-2R | 5' -CGCCGAATTCATGGGGTCACAAGT -3' | EcoR| | Â |
CA16-VP4-2F | 5'- TATCTCGAGTTACTTGAGCGGCGGG -3' | Xho| | 229 |