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Table 4 Primers used for cloning and sequencing

From: Comparing Enterovirus 71 with Coxsackievirus A16 by analyzing nucleotide sequences and antigenicity of recombinant proteins of VP1s and VP4s

primers

sequences

fragments (bp)

EV71-VP1-1F

5'-TGAAGTTRTGYAAGGATGC-3'

 

EV71-VP1-1R

5'-CCACTCTAAAATTRCCCAC-3'

993

EV71-VP4-1F

5'-CTACTTTGGGTGTCCGTGTT-3'

 

EV71-VP4-1R

5'-GGGAACTTCCAGTACCATCC-3'

655

CA16-VP1-1F

5'-ACTATGCAAGGACACWGAG -3'

 

CA16-VP1-1R

5'- CAGTGGTGGAAGAGACTAAA-3'

1076

CA16-VP4-1F

5'- GGCTGCTTATGGTGACAA-3'

 

CA16-VP4-1R

5'- CATGGGAGCTATGGTGAC-3'

1090

  1. F referred as forward primer and R referred as reverse primer.
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BMC Microbiology

ISSN: 1471-2180

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