Figure 1

Phylogenetic analysis based on EV71 vp1s (A), EV71 vp4s (B), CA16 vp1s (C) and CA16 vp4s (D). The unrooted phylogenetic trees were generated by the neighbor-joining method on the basis of a multiple alignment of the nucleotide sequences of EV71 vp1s, EV71 vp4s, CA16 vp1s and CA16 vp4s. The sequences in the dendrograms marked by red circle (○), green triangle (Δ) and blue square (□) were isolated in this research (additional file 2) while other sequences were obtained from GenBank (additional file 1). CA16 strain G-10 was used as an outgroup in Figure 1A and Figure 1B while EV71 strain BrCr was used as an outgroup in Figure 1C and Figure 1D.