Figure 3
From: Biofilm and planktonic pneumococci demonstrate disparate immunoreactivity to human convalescent sera
Identification of immunogenic proteins enhanced during pneumococcal biofilm growth. A) Immunoblots of planktonic and biofilm S. pneumoniae cell lysates separated by 2DGE and probed with pooled human convalescent sera. B) Coomassie blue stained 2DGE gel of biofilm proteins showing the 20 immunogenic protein spots (circled in red) selected for analysis by MALDI-TOF. The corresponding spots detected with convalescent sera are circled in the biofilm immunoblot in panel A.