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Table 1 PCR primers used

From: Assessing diversity of the female urine microbiota by high throughput sequencing of 16S rDNA amplicons

Primer

Sequence (5'→3')

16S rDNA region

Product size

Reference

A2+V1 F

GCCTCCCTCGCGCCATCAGAGAGTTTGATCMTGGCTCAG

V1V2

392 bp3

[32]

B2+V2 R

GCCTTGCCAGCCCGCTCAGCYNACTGCTGCCTCCCGTAG

8-3611

  

A2+1061R

GCCTCCCTCGCGCCATCAGCRRCACGAGCTGACGAC

V6

316 bp3

[33]

B2 +784F

GCCTTGCCAGCCCGCTCAGAGGATTAGATACCCTGGTA

784-10611

  
  1. The table contains primer name, sequence (hypervariable specific sequence in bold font), 16S rDNA region covered, product size and references for the primers used in this study.
  2. 1 Coordinates are given relative to the 1542 bp E. coli K12 16S rDNA sequence.
  3. 2A and B primer: corresponds to 454-adaptor sequences from the amplicon pyrosequencing protocol for GS FLX http://www.my454.com/downloads/protocols/Guide_To_Amplicon_Sequencing.pdf[101], p. 7.
  4. 3Product size includes the primer sequences.
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BMC Microbiology

ISSN: 1471-2180

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