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Table 3 Transformation by plasmids of the moderately thermophilic Streptomyces 2C and 4F

From: Development of a gene cloning system in a fast-growing and moderately thermophilic Streptomyces species and heterologous expression of Streptomyces antibiotic biosynthetic gene clusters

Plasmids Replicons Hosts Transformation frequency (transformants/μg DNA)
    2C 4F
pIJ702 pIJ101 S. lividans ZX7 1.3 × 106 3 × 102
pIJ702 pIJ101 2C 2.9 × 106 8 × 101
pIJ702 pIJ101 4F 1.4 × 105 1.2 × 105
pCWH1 pTSC1 E. coli DH5α 1.3 × 103 2 × 101
pZR51 pFRL2 E. coli DH5α 8.2 × 103 1 × 101
pZR115 pFP1 E. coli DH5α 1 × 102 2 × 101
pZR10 pFP11 E. coli DH5α 2 × 102 1