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Table 3 Transformation by plasmids of the moderately thermophilic Streptomyces 2C and 4F

From: Development of a gene cloning system in a fast-growing and moderately thermophilic Streptomyces species and heterologous expression of Streptomyces antibiotic biosynthetic gene clusters

Plasmids

Replicons

Hosts

Transformation frequency (transformants/μg DNA)

   

2C

4F

pIJ702

pIJ101

S. lividans ZX7

1.3 × 106

3 × 102

pIJ702

pIJ101

2C

2.9 × 106

8 × 101

pIJ702

pIJ101

4F

1.4 × 105

1.2 × 105

pCWH1

pTSC1

E. coli DH5α

1.3 × 103

2 × 101

pZR51

pFRL2

E. coli DH5α

8.2 × 103

1 × 101

pZR115

pFP1

E. coli DH5α

1 × 102

2 × 101

pZR10

pFP11

E. coli DH5α

2 × 102

1

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BMC Microbiology

ISSN: 1471-2180

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