Table 2 Plasmids used in this study
Plasmids | Genotype or description | Source or reference |
|---|---|---|
pTSC1 | A 6996-bp plasmid of strain X4-3 | This work |
pTSC2 | A 7.5-kb plasmid of strain X3-3 | This work |
pTSC3 | A 50-kb plasmid of strain T6-1-4 | This work |
pTSL1 | A 16-kb linear plasmid of strain T6-1-4 | This work |
pSP72 | amp colEI-ori | Life Technologies, Inc |
pBluescript II SK | amp colEI-ori lacZ | Stratagene, Inc |
pQC156 | A 2.6-kb BclI-fragment of melC/tsr cloned in pSP72 (BglII) | [46] |
pCWH1 | A 7-kb KpnI fragment of pTSC1 cloned in pQC156 | This work |
pCWH100 | A 7-kb KpnI fragment of pTSC1 cloned in pBluescript II SK | This work |
pIJ702 | melC tsr pIJ101 origin | [31] |
pZR10 | A 8.9-kb Sau3A1-fragment of pFP11 origin cloned in pQC156 | [33] |
pZR115 | A 4.1-kb Sau3A1-fragment of pFP1 origin cloned in pQC156 | [33] |
pZR205 | Two fragments (PCR) of SLP1 rep/imp cloned in pQC156 | [33] |
pZR51 | A 2.2-kb HindIII fragment of pFRL2 origin cloned into pQC156 | [32] |
pHAQ61 | A 2.9-kb fragment of SAP1 origin cloned in pQC156 | Zhang and Qin, unpublished data |
pYQ40 | A 2-kb fragment of SCP2 origin cloned in pQC156 | Yang and Qin, unpublished data |
pGP9 | A 4.1-kb EcoRI/BglII fragment of pSHK1 origin cloned in pQC156 | [32] |
pSET152 | Streptomyces phage φC31-derived integration vector, aprr | [38] |
pHAQ31 | amp colEI-ori cos melC tsr | [47] |
Cosmid N7-85 | pHAQ31 (BamHI) containing c. 33 kb sequence (5510413-5543521 bp) from S. coelicolor A3(2) | This work |
pCWH74 | A 2.6-kb XbaI/NheI fragment containing the phiC31 integrase gene cloned in a pHAQ31-derived cosmid containing the actinorhodin biosynthetic gene cluster | This work |
024CAO-3 | The anthramycin biosynthetic gene cluster cloned into a cosmid CAO2 | [22] |