| |
Overexpression levels* and no. of isolates** showing gene overexpression
|
|---|
| |
½ CIP MIC
|
½ EtBr MIC
|
|---|
| | |
EtBrCW-
|
EtBrCW+
| |
EtBrCW-
|
EtBrCW+
|
|---|
|
Gene
|
ATCC25923
|
isolates
|
isolates
|
ATCC25923
|
isolates
|
isolates
|
|---|
| | |
(n = 4)
|
(n = 6)
| |
(n = 4)
|
(n = 6)
|
|---|
|
norA
|
-
|
-
|
-
|
4.51 ± 0.77
|
-
|
-
|
| | |
0
|
0
| |
0
|
0
|
|
norB
|
13.80 ± 6.50
|
5.43 ± 2.39
|
5.47 ± 0.19
|
7.07 ± 2.78
|
5.33 ± 0.73
|
-
|
| | |
2
a, b
|
1
e
| |
1
a
|
0
|
|
norC
|
-
|
-
|
4.92 ± 0.00
|
5.89 ± 0.71
|
4.99 ± 1.51
|
-
|
| | |
0
|
1
e
| |
1
a
|
0
|
|
mepA
|
-
|
-
|
8.59 ± 0.59
|
3.90 ± 0.13
|
5.94 ± 1.02
|
-
|
| | |
0
|
1
f
| |
1
a
|
0
|
|
mdeA
|
-
|
4.97 ± 0.68
|
-
|
3.96 ± 2.10
|
-
|
4.15 ± 1.12
|
| | |
1
c
|
0
| |
0
|
1
d
|
|
smr
|
n.a.
|
n.a.
|
-
|
n.a.
|
n.a.
|
7.66 ± 3.66
|
| | | |
0
| | |
1
f
|
- * Gene expression was measured in the presence of ciprofloxacin and EtBr relatively to the drug-free condition. The results are expressed in terms of the mean ± standard deviation of at least three independent assays performed with independently extracted RNAs and correspond to the range of values obtained for isolates showing overexpression of that gene. **The numbers in bold correspond to the number of isolates overexpressing that gene: a isolate SM2; b SM3; c SM5; dSM25; e SM50; f SM52. Overexpression was considered for values ≥4 [10]. (-): no overexpression was detected; n.a.: not applicable.
