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Figure 1 | BMC Microbiology

Figure 1

From: Activation of PI3K/AKT and ERK MAPK signal pathways is required for the induction of lytic cycle replication of Kaposi's Sarcoma-associated herpesvirus by herpes simplex virus type 1

Figure 1

Either JAK1/STAT3 or JAK1/STAT6 signal pathway does not mediate HSV-1-induced KSHV replication. (A) Western blot analysis for phosphorylation of STAT3 and STAT6. BCBL-1 cells were infected with Mock (M) or HSV-1 (H) for 3, 6, 12, and 24 h. Cells were collected and cell lysates were subjected to SDS-PAGE, transferred to membrane, and then immunoblotted with the indicated antibodies. (B) RT-qPCR was used to detect relative quantities of ORF26 mRNA in STAT3-DN (pST3-DN) or control vector transfected and HSV-1 infected BCBL-1 cells as indicated. ** p < 0.01 and *** p < 0.001 for Student's t-test versus Mock + pMSCV group; n.s., not significant for Student's t-test versus HSV-1 + pMSCV group. (C) RT-qPCR was used to detect relative quantities of ORF26 mRNA in STAT6-DN (pST6-DN) or control vector transfected and HSV-1 infected BCBL-1 cells as indicated. ** p < 0.01 and *** p < 0.001 for Student's t-test versus Mock + pRed group; n.s., not significant for Student's t-test versus HSV-1 + pRed group.

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BMC Microbiology

ISSN: 1471-2180

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