Figure 4
From: Arcanolysin is a cholesterol-dependent cytolysin of the human pathogen Arcanobacterium haemolyticum
Overexpression and purification of His-ALN. Whole-cell lysates of IPTG-induced cultures of DH5αMCR(pTrcHis B) (lane 1) and DH5αMCR (pBJ51) (lane 2) and 500 ng purified His-ALN (lane 3) were subjected to SDS-PAGE. Separated proteins were stained with Coomassie brilliant blue (a) or were transferred to nitrocellulose by Western blotting and immunostained with 1/5000 rabbit pre-immune serum (b) or rabbit anti-His-ALN (c). The position of the ~64 kDa His-ALN band is indicated by the arrow. Molecular mass markers (kDa) are indicated on the left.