Figure 1
From: Arcanolysin is a cholesterol-dependent cytolysin of the human pathogen Arcanobacterium haemolyticum
Map of the A. haemolyticum aln region and presence of aln in clinical isolates. (a) Map of the aln gene region of strain ATCC 9345 (= DSM20595 = 11018). The open arrows indicate the gene and the direction of transcription. Gene names are given and the number indicates the %G+C of the gene. A bar indicating 1 kb is shown on the right. (b) DNA dot hybridization of genomic DNA from A. haemolyticum strains with an aln-specific probe. Genomic DNA from 52 A. haemolyticum isolates and T. pyogenes BBR1, as a negative control (~500 ng each), was spotted onto a nylon membrane and hybridized with aln-specific probe under high stringency conditions. A. haemolyticum ATCC9345 DNA is in the second from last spot. T. pyogenes BBR1 DNA is in the last spot.