Map of the A. haemolyticum aln region and presence of aln in clinical isolates. (a) Map of the aln gene region of strain ATCC 9345 (= DSM20595 = 11018). The open arrows indicate the gene and the direction of transcription. Gene names are given and the number indicates the %G+C of the gene. A bar indicating 1 kb is shown on the right. (b) DNA dot hybridization of genomic DNA from A. haemolyticum strains with an aln-specific probe. Genomic DNA from 52 A. haemolyticum isolates and T. pyogenes BBR1, as a negative control (~500 ng each), was spotted onto a nylon membrane and hybridized with aln-specific probe under high stringency conditions. A. haemolyticum ATCC9345 DNA is in the second from last spot. T. pyogenes BBR1 DNA is in the last spot.