Table 1 Strains and plasmid used in this study.
Strains or plasmids | Relevant phenotype or genotype | Reference |
|---|---|---|
Strains | ||
S. thermophilus | ||
CNRZ368 | Wild-type strain carrying ICESt1 | INRA-CNRZ |
CNRZ385 | Wild-type strain carrying ICESt3 | INRA-CNRZ |
CNRZ368ΔICESt1 | Wild-type strain cured from its ICESt1 resident element | X. Bellanger pers. com. |
LMG18311 ICESt3cat | Wild-type strain carrying ICESt3 tagged with the cat gene inserted in the pseudogene Ψorf385J, Cmr | [10] |
CNRZ368 ICESt3cat | CNRZ368ΔICESt1 strain carrying ICESt3cat, Cmr | This work |
E. coli | ||
DH5α | supE44 lacU169 (φ80 lacZ M15) hsdR17 endA1 gyrA96 thi-1 relA1 | [33] |
Plasmid | ||
pSL1180 | 3, 4 kb, replication origin from pBR322, Ampr | Amersham |