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Table 1 Strains and plasmid used in this study.

From: Differential regulation of two closely related integrative and conjugative elements from Streptococcus thermophilus

Strains or plasmids Relevant phenotype or genotype Reference
Strains   
   S. thermophilus   
CNRZ368 Wild-type strain carrying ICESt1 INRA-CNRZ
CNRZ385 Wild-type strain carrying ICESt3 INRA-CNRZ
CNRZ368ΔICESt1 Wild-type strain cured from its ICESt1 resident element X. Bellanger pers. com.
LMG18311 ICESt3cat Wild-type strain carrying ICESt3 tagged with the cat gene inserted in the pseudogene Ψorf385J, Cmr [10]
CNRZ368 ICESt3cat CNRZ368ΔICESt1 strain carrying ICESt3cat, Cmr This work
   E. coli   
DH5α supE44 lacU169 (φ80 lacZ M15) hsdR17 endA1 gyrA96 thi-1 relA1 [33]
Plasmid   
   pSL1180 3, 4 kb, replication origin from pBR322, Ampr Amersham
  1. Abbreviations: Cmr, chloramphenicol resistance, Ampr: ampicillin resistance.