Figure 2
Transcriptional analysis of the arp2 / orfM region of ICE St3. (A) Schematic representation of the arp2/orfM intergenic region of ICESt3. Primers used for PCR analysis are represented by triangles and promoters are represented by angled arrows. (B) RT-PCR mapping Pcr promoter of ICESt3. Amplicons are generated with primers mentioned above the gels on genomic DNA (gDNA) or cDNA synthesized from RNA extracted from cells in exponential growth phase (expo0.6). Amplicon size is given on the left. Results were identical for three independent biological replicates. (C) RT-PCR mapping Parp2 promoter of ICESt3. Amplicons are generated with primers mentioned on the left of the gels on genomic DNA (gDNA) or cDNA synthesized from RNA extracted from exponential growth phase (expo0.6) and stationary phase (stat) cells. The transcriptional activity upstream from the Parp2 promoter was detected during stationary phase. Results were identical for three independent biological replicates.