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Table 4 Gene Ontology (GO) functional enrichment analysis of the flavivirus-targeted human proteins

From: Flavivirus NS3 and NS5 proteins interaction network: a high-throughput yeast two-hybrid screen

Ontology Description GO term p-value Associated proteins
Molecular function RNA binding GO:0003723 **** EIF5A, HNRPF, HNRPH3, ILF3, MATR3, MRPL20, PABPC1, PPRC1, PRKRA, RNUXA, RPS20, SSB, TAF15, TRIM21, SNRPA, XPO1, ZCCHC17
  Structural constituent of cytoskeleton GO:0005200 ** ACTB, ACTG1, BICD1, KRT19, VIM
  Nuclear localization sequence binding GO:0008139 ** KPNB1, NFKBIA
  Transcription factor binding GO:0008134 * ARNTL, CAMTA2, HNRNPF, KAT5, MDF1, MED4, NFKBIA
  Transcription corepressor activity GO:0003714 * ATN1, ENO1, RNF12, SIAH2, TSG101
Cellular component Cytoskeleton GO:0005856 **** ACTA2, ACTB, ACTG1, ACTG2, APBB1IP, AXIN1, BICD1, CASP8, CCDC99, CEP250, CEP290, CEP63, CHD3, CLIP1, DNM2, FHL2, GOPC, KIF3B, KRT19, LMNA, MLPH, MYH9, PDE4DIP, TRAF4, TYK2, VIM
  Ribonucleoprotein complex GO:0030529 ** ACTB, HNRNPF, HNRNPH3, ILF3, MRPL20, PABPC1, RPS20, SSB, SNRPA, ZCCHC17
  H4/H2A histone acetyltransferase complex GO:0043189 ** ACTB, KAT5
Biological process Intracellular transport GO:0046907 *** AXIN1, BICD1, DNM2, EIF5A, GGA1, GOPC, KIF3B, KPNB1, MLPH, NFKBIA, NRBP1, OPTN, RNUXA, TOM1L1, TSG101, XPO1
  Regulation of type I interferon-mediated signaling pathway GO:0060338 *** HSP90AB1, IFNAR2, STAT2, TYK2
  Regulation of innate immune response GO:0045088 ** HSP90AB1, IFNAR2, NFKBIA, TRAFD1, TYK2
  Viral reproductive process GO:0022415 ** KPNB1, PPIA, RPS20, SMARCB1, TSG101, XPO1
  Post-Golgi vesicle-mediated transport GO:0006892 * DNM2, GOPC, OPTN
  Nuclear transport GO:0051169 * AXIN1, EIF5A, KPNB1, NFKBIA, RNUXA
  1. We assigned their GO features to the human proteins identified in our screen (literature plus Y2H). We then determined if these features were over-represented in comparison with the complete list of the annotated human proteins. The description of the GO enriched term (column 2), the corresponding GO identifier (column 3), the significativity of the functional enrichment test (**** p-value < = 0.0001, *** p-value < = 0.001, ** p-value < = 0.01, * p-value < 0, 05) and the associated proteins (colum 5) are given in table 4. The three GO subcategories are presented: molecular function, cellular component and biological process.