Flavivirus NS3 and NS5 proteins interaction network: a high-throughput yeast two-hybrid screen

BMC Microbiology

Table 4 Gene Ontology (GO) functional enrichment analysis of the flavivirus-targeted human proteins

Ontology	Description	GO term	p-value	Associated proteins
Molecular function	RNA binding	GO:0003723	****	EIF5A, HNRPF, HNRPH3, ILF3, MATR3, MRPL20, PABPC1, PPRC1, PRKRA, RNUXA, RPS20, SSB, TAF15, TRIM21, SNRPA, XPO1, ZCCHC17
	Structural constituent of cytoskeleton	GO:0005200	**	ACTB, ACTG1, BICD1, KRT19, VIM
	Nuclear localization sequence binding	GO:0008139	**	KPNB1, NFKBIA
	Transcription factor binding	GO:0008134	*	ARNTL, CAMTA2, HNRNPF, KAT5, MDF1, MED4, NFKBIA
	Transcription corepressor activity	GO:0003714	*	ATN1, ENO1, RNF12, SIAH2, TSG101
Cellular component	Cytoskeleton	GO:0005856	****	ACTA2, ACTB, ACTG1, ACTG2, APBB1IP, AXIN1, BICD1, CASP8, CCDC99, CEP250, CEP290, CEP63, CHD3, CLIP1, DNM2, FHL2, GOPC, KIF3B, KRT19, LMNA, MLPH, MYH9, PDE4DIP, TRAF4, TYK2, VIM
	Ribonucleoprotein complex	GO:0030529	**	ACTB, HNRNPF, HNRNPH3, ILF3, MRPL20, PABPC1, RPS20, SSB, SNRPA, ZCCHC17
	H4/H2A histone acetyltransferase complex	GO:0043189	**	ACTB, KAT5
Biological process	Intracellular transport	GO:0046907	***	AXIN1, BICD1, DNM2, EIF5A, GGA1, GOPC, KIF3B, KPNB1, MLPH, NFKBIA, NRBP1, OPTN, RNUXA, TOM1L1, TSG101, XPO1
	Regulation of type I interferon-mediated signaling pathway	GO:0060338	***	HSP90AB1, IFNAR2, STAT2, TYK2
	Regulation of innate immune response	GO:0045088	**	HSP90AB1, IFNAR2, NFKBIA, TRAFD1, TYK2
	Viral reproductive process	GO:0022415	**	KPNB1, PPIA, RPS20, SMARCB1, TSG101, XPO1
	Post-Golgi vesicle-mediated transport	GO:0006892	*	DNM2, GOPC, OPTN
	Nuclear transport	GO:0051169	*	AXIN1, EIF5A, KPNB1, NFKBIA, RNUXA

We assigned their GO features to the human proteins identified in our screen (literature plus Y2H). We then determined if these features were over-represented in comparison with the complete list of the annotated human proteins. The description of the GO enriched term (column 2), the corresponding GO identifier (column 3), the significativity of the functional enrichment test (**** p-value < = 0.0001, *** p-value < = 0.001, ** p-value < = 0.01, * p-value < 0, 05) and the associated proteins (colum 5) are given in table 4. The three GO subcategories are presented: molecular function, cellular component and biological process.

ISSN: 1471-2180