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Table 2 Effect of mAb treatment on in vitro attachment and aggregation of WB Giardia trophozoites

From: Immunodominant proteins α-1 giardin and β-giardin are expressed in both assemblages A and B of Giardia lamblia

  Trophozoite adhesion* Trophozoite aggregation
  0 hours 2 hours 4 hours 0 hours 2 hours 4 hours
Without mAb 20 ± 2 19 ± 2 20 ± 2 - - -
Anti-HA-mAb 20 ± 2 19 ± 2 22 ± 2 - - -
Anti-VSP-mAb 21 ± 2 15 ± 2 11 ± 2 - ++ ++++
G3G10-mAb 19 ± 2 20 ± 2 18 ± 2 - - -
  1. *values are an average of 10 random vertical scans of well surface.
  2. The dash (-) indicates no effects. (+) indicates between 4-6 clusters of grouped cells. (++) indicates between 8-10 clusters of grouped cells. (+++) indicates between 15-18 clusters of grouped cells. (++++) indicates more than 20 clusters of grouped cells.
  3. Assays were performed in triplicate and scored by persons unaware of the contents of the wells.