Immunolocalization of α-1 giardin Giardia trophozoites. (A) Reactivity of G3G10 mAb on WB and GS Giardia trophozoites was determined by indirect immunofluorescence in permeabilized (upper panels) and non-permeabilized (lower panels) trophozoites. The arrowheads show the paraflagellar dense rods and the arrows indicate the bare area. Scale bar: 10 μm. (B) Reactivity of G3G10 in permeabilized trophozoites of WB clone C6, WB clone A6, Portland-1 and P-15 strains. Scale bar: 10 μm.