Binding of His-PhbF to PHB granules. Purified His-PhbF was incubated with polyhydroxybutyrate (PHB) granules as described in Material and Methods. Lane 1: 2 μg of purified His-PhbF; lane 2: non-adsorbed protein; lanes 3 and 4: washing buffer; lane 5: PHB-adsorbed protein after elution with 2% (m/v) SDS, 10% (m/v) glycerol and 5% (m/v) β-mercaptoethanol at 90°C for five minutes; lane 6: PHB-granule control treated with 2% (m/v) SDS, 10% (m/v) glycerol and 5% (m/v) β-mercaptoethanol at 90°C for five minutes. MW: molecular weight markers (kDa). Arrow indicates His-PhbF. The SDS-PAGE gel was stained with Coomassie blue.