Table 2 Plasmids used in this study
Plasmid | Characteristics | Oligonucleotides† | Reference or source |
|---|---|---|---|
pGh9 | Thermosensitive plasmid carrying erythromycin resistance | [46] | |
pGEM-T easy | Promega | ||
PCR-Blunt II-TOPO | Invitrogen | ||
pET28a | Novagen | ||
pBM02 | pUC18 derivative carrying CRL264 replicon, Pcit (promoter) and chloramphenicol resistance | [28] | |
pTCV-lac | Promoterless vector which allows lacZ fusion construction | [26] | |
pmCitO | pGh9 derivative carrying a 500 bp citO internal fragment | fcitOU, fcitOL | [6] |
pET-CcpA | pET28a derivative expressing His6-CcpA | Ef-ccpAU, Ef-ccpAL | This study |
pCitO | pBM02 derivative for expressing CitO in E. faecalis | [6] | |
pTCV-PcitHO | EfHpromU, EfDpromL | [6] | |
pTCV-PcitCL | EfHpromU, EfDpromL | [6] | |
pTCV-PcitHO-C 1 C 2 | EfHpromU, EfbsPcitN | This study | |
pTCV-PcitHO-C 1 C 2M | EfHpromU, EfbsPcitN | This study | |
pTCV-PcitHO-C 2 C 3 | EfbscitN, Efint_Lo | This study | |
pTCV-PcitHO-C 2M C 3 | EfbscitN, Efint_Lo | This study | |
pTCV-PcitHO-C 2 C 3M | EfbscitN, Efint_Lo | This study | |
pTCV-PcitCL-C 2 C 3 | EfbscitN, Efint_Lo | This study | |
pTCV-PcitCL-C 2 C 3 | EfbscitN, Efint_Lo | This study | |
pTCV-PcitCL-C 2 C 3M | EfbscitN, Efint_Lo | This study | |
pTCV-PcitCL-C 2M C 3 | EfbscitN, Efint_Lo | This study | |
EfbscitN, Efint_Lo | This study |