Skip to main content

Advertisement

Table 2 Plasmids used in this study

From: CcpA represses the expression of the divergent cit operons of Enterococcus faecalis through multiple cresites

Plasmid Characteristics Oligonucleotides Reference or source
pGh9 Thermosensitive plasmid carrying erythromycin resistance   [46]
pGEM-T easy    Promega
PCR-Blunt II-TOPO    Invitrogen
pET28a    Novagen
pBM02 pUC18 derivative carrying CRL264 replicon, Pcit (promoter) and chloramphenicol resistance   [28]
pTCV-lac Promoterless vector which allows lacZ fusion construction   [26]
pmCitO pGh9 derivative carrying a 500 bp citO internal fragment fcitOU, fcitOL [6]
pET-CcpA pET28a derivative expressing His6-CcpA Ef-ccpAU, Ef-ccpAL This study
pCitO pBM02 derivative for expressing CitO in E. faecalis   [6]
pTCV-PcitHO   EfHpromU, EfDpromL [6]
pTCV-PcitCL   EfHpromU, EfDpromL [6]
pTCV-PcitHO-C 1 C 2   EfHpromU, EfbsPcitN This study
pTCV-PcitHO-C 1 C 2M   EfHpromU, EfbsPcitN This study
pTCV-PcitHO-C 2 C 3   EfbscitN, Efint_Lo This study
pTCV-PcitHO-C 2M C 3   EfbscitN, Efint_Lo This study
pTCV-PcitHO-C 2 C 3M   EfbscitN, Efint_Lo This study
pTCV-PcitCL-C 2 C 3   EfbscitN, Efint_Lo This study
pTCV-PcitCL-C 2 C 3   EfbscitN, Efint_Lo This study
pTCV-PcitCL-C 2 C 3M   EfbscitN, Efint_Lo This study
pTCV-PcitCL-C 2M C 3   EfbscitN, Efint_Lo This study
   EfbscitN, Efint_Lo This study
  1. †Oligonucleotide sequences are indicated in Table 3.