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Table 1 Bacterial strains and plasmids used in this study

From: The rgg 0182 gene encodes a transcriptional regulator required for the full Streptococcus thermophilusLMG18311 thermal adaptation

Strains and plasmids Genotype/phenotype/source Origin or reference
Streptococcus thermophilus
LMG18311 Wild-type; isolated from yogurt. BCCM LMG
Δrgg 0182 Derivative of LMG18311 carrying a 699 bp deletion in the rgg 0182 gene This study
LMG18311 blp::P pep0182 -luxAB LMG18311 containing a P pep0182-luxAB fusion at the blp locus This study
LMG18311 blp::P shp0182 -luxAB LMG18311 containing a P shp0182-luxAB fusion at the blp locus This study
Δrgg 0182 blp::P pep0182 -luxAB Δrgg 0182 containing a P pep0182-luxAB fusion at the blp locus This study
Δrgg 0182 blp::P shp0182 -luxAB Δrgg 0182 containing a P shp0182-luxAB fusion at the blp locus This study
Escherichia coli
EC101 supE hsd-5 thi Δ(lac-proAB) F'(traD6 proAB+lacIqlacZΔM15) repA+, derivative of TG1 strain [37, 38]
EC101 pG9Δrgg 0182 Derivative of EC101 containing pG9Δrgg 0182 This study
C41(DE3) Derived from BL21(DE3) (F- ompT hsdSB(rB- mB-) gal dcm met (DE3); Novagen); at least one uncharacterized mutation [39]
C41(DE3) pET15b::rgg 0182 Derivative of C41(DE3) containing pET15b::rgg 0182 This study
Plasmid
pG+host9 Thermosensitive plasmid replication origin from pVE6002, used for gene replacement; EryR [36]
pG9Δrgg 0182 Derivative of pG+host9 carrying a 699 bp (from nucleotide 82 to 783) deletion for rgg0182 This study
pGICB004 Thermosensitive plasmid, Emr; used for the construct of transcriptional fusions. Allow the integration of the luxAB genes of Photorabdus luminescens at the blp locus of the S. thermophilus chromosome [9]
pGICB004::P pep0182 Derivative of pGICB004 used to introduce a P pep0182 -luxAB transcriptional fusion at the blp locus This study
pGICB004::P shp0182 Derivative of pGICB004 used to introduce a P shp0182 -luxAB transcriptional fusion at the blp locus This study
pET15b Expression vector for N-terminal His6-tagged fusion; AmpR Novagen
pET15b::rgg 0182 Derivative of pET15b carrying a 864 bp insert coding a Rgg0182 His6-tagged protein This study
  1. BCCM Belgian Coordinated Collections of Microorganisms, LMG Laboratory of Microbiology and Genetics, University of Gent