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Table 1 Bacterial strains and plasmids used in this study

From: The rgg 0182 gene encodes a transcriptional regulator required for the full Streptococcus thermophilusLMG18311 thermal adaptation

Strains and plasmids

Genotype/phenotype/source

Origin or reference

Streptococcus thermophilus

LMG18311

Wild-type; isolated from yogurt.

BCCM LMG

Δrgg 0182

Derivative of LMG18311 carrying a 699 bp deletion in the rgg 0182 gene

This study

LMG18311 blp::P pep0182 -luxAB

LMG18311 containing a P pep0182-luxAB fusion at the blp locus

This study

LMG18311 blp::P shp0182 -luxAB

LMG18311 containing a P shp0182-luxAB fusion at the blp locus

This study

Δrgg 0182 blp::P pep0182 -luxAB

Δrgg 0182 containing a P pep0182-luxAB fusion at the blp locus

This study

Δrgg 0182 blp::P shp0182 -luxAB

Δrgg 0182 containing a P shp0182-luxAB fusion at the blp locus

This study

Escherichia coli

EC101

supE hsd-5 thi Δ(lac-proAB) F'(traD6 proAB+lacIqlacZΔM15) repA+, derivative of TG1 strain

[37, 38]

EC101 pG9Δrgg 0182

Derivative of EC101 containing pG9Δrgg 0182

This study

C41(DE3)

Derived from BL21(DE3) (F- ompT hsdSB(rB- mB-) gal dcm met (DE3); Novagen); at least one uncharacterized mutation

[39]

C41(DE3) pET15b::rgg 0182

Derivative of C41(DE3) containing pET15b::rgg 0182

This study

Plasmid

pG+host9

Thermosensitive plasmid replication origin from pVE6002, used for gene replacement; EryR

[36]

pG9Δrgg 0182

Derivative of pG+host9 carrying a 699 bp (from nucleotide 82 to 783) deletion for rgg0182

This study

pGICB004

Thermosensitive plasmid, Emr; used for the construct of transcriptional fusions. Allow the integration of the luxAB genes of Photorabdus luminescens at the blp locus of the S. thermophilus chromosome

[9]

pGICB004::P pep0182

Derivative of pGICB004 used to introduce a P pep0182 -luxAB transcriptional fusion at the blp locus

This study

pGICB004::P shp0182

Derivative of pGICB004 used to introduce a P shp0182 -luxAB transcriptional fusion at the blp locus

This study

pET15b

Expression vector for N-terminal His6-tagged fusion; AmpR

Novagen

pET15b::rgg 0182

Derivative of pET15b carrying a 864 bp insert coding a Rgg0182 His6-tagged protein

This study

  1. BCCM Belgian Coordinated Collections of Microorganisms, LMG Laboratory of Microbiology and Genetics, University of Gent