Strains and plasmids | Genotype/phenotype/source | Origin or reference |
---|---|---|
Streptococcus thermophilus | ||
LMG18311 | Wild-type; isolated from yogurt. | BCCM LMG |
Δrgg 0182 | Derivative of LMG18311 carrying a 699 bp deletion in the rgg 0182 gene | This study |
LMG18311 blp::P pep0182 -luxAB | LMG18311 containing a P pep0182-luxAB fusion at the blp locus | This study |
LMG18311 blp::P shp0182 -luxAB | LMG18311 containing a P shp0182-luxAB fusion at the blp locus | This study |
Δrgg 0182 blp::P pep0182 -luxAB | Δrgg 0182 containing a P pep0182-luxAB fusion at the blp locus | This study |
Δrgg 0182 blp::P shp0182 -luxAB | Δrgg 0182 containing a P shp0182-luxAB fusion at the blp locus | This study |
Escherichia coli | ||
EC101 | supE hsd-5 thi Δ(lac-proAB) F'(traD6 proAB+lacIqlacZΔM15) repA+, derivative of TG1 strain | |
EC101 pG9Δrgg 0182 | Derivative of EC101 containing pG9Δrgg 0182 | This study |
C41(DE3) | Derived from BL21(DE3) (F- ompT hsdSB(rB- mB-) gal dcm met (DE3); Novagen); at least one uncharacterized mutation | [39] |
C41(DE3) pET15b::rgg 0182 | Derivative of C41(DE3) containing pET15b::rgg 0182 | This study |
Plasmid | ||
pG+host9 | Thermosensitive plasmid replication origin from pVE6002, used for gene replacement; EryR | [36] |
pG9Δrgg 0182 | Derivative of pG+host9 carrying a 699 bp (from nucleotide 82 to 783) deletion for rgg0182 | This study |
pGICB004 | Thermosensitive plasmid, Emr; used for the construct of transcriptional fusions. Allow the integration of the luxAB genes of Photorabdus luminescens at the blp locus of the S. thermophilus chromosome | [9] |
pGICB004::P pep0182 | Derivative of pGICB004 used to introduce a P pep0182 -luxAB transcriptional fusion at the blp locus | This study |
pGICB004::P shp0182 | Derivative of pGICB004 used to introduce a P shp0182 -luxAB transcriptional fusion at the blp locus | This study |
pET15b | Expression vector for N-terminal His6-tagged fusion; AmpR | Novagen |
pET15b::rgg 0182 | Derivative of pET15b carrying a 864 bp insert coding a Rgg0182 His6-tagged protein | This study |