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Table 2 In silico prediction of A-domain specificity for Ps1448a pyoverdine side chain NRPS

From: Characterization of pyoverdine and achromobactin in Pseudomonas syringae pv. phaseolicola 1448a

A domain

8 residue signature alignment

Identity of best match

TSVM prediction congruent?

1923

DGEDHGTV

| | |:|

DAESIGSV

BacB-M1-Lys bacitracin synthetase 2

No: val = leu = ile = abu = iva-like specificity

1924 mod1

DLTKIGHV

||||:||:

DLTKVGHI

SrfAB-M2-Asp surfactin synthetase B

Yes:

asp = asn = glu = gln = aad-like specificity

1924 mod2

DFWNIGMV

||||||||

DFWNIGMV

PvdD-M2-Thr

pyoverdine synthetase

Yes:

thr = dht-like specificity

1925 mod1

DFWNIGMV

||||||||

DFWNIGMV

PvdD-M2-Thr

pyoverdine synthetase

Yes:

thr = dht-like specificity

1925mod2

DVWHVSLI

||||||||

DVWHVSLI

PvdJ-M1-Ser pyoverdine synthetase

Yes:

ser-like specificity

1926 mod1

DLTKIGHV

||||:||:

DLTKVGHI

SrfAB-M2-Asp surfactin synthetase B

Yes:

asp = asn = glu = gln = aad-like specificity

1926 mod2

DVWHVSLI

||||||||

DVWHVSLI

PvdJ-M1-Ser pyoverdine synthetase

Yes:

ser-like specificity