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Table 2 Previously published interactions among lambda proteins

From: The protein interaction map of bacteriophage lambda

  interacting λ proteins notes ref#
head
1 A Nu1 A (N-term) - Nu1 (C-term) [3234]
2 A B A (C-term) - B (= portal) [32, 35]
3 A FI Genetic evidence [21]
4 FI E Genetic evidence [22]
5 Nu3 B Nu3 required for B incorporation into procapsid [36]
6 W B   [37, 38]
7 W FII W required for FII binding, FII connects head to tail [37, 39]
8 B B 12-mer (22 aa removed from B N-term) [40, 41]
9 C E Covalent PPI (in virion?) [42, 43]
10 C B   [44]
11 B E copurify in procapsid [45]
12 C Nu3 C may degrade Nu3 (before DNA packaging) [4547]
13 D D Capsid vertices, D forms trimers [4850]
14 E E Main capsid protein [20, 51, 52]
15 D E   [20, 51, 52]
  Nu3 Nu3 Nu3 multimer unpublished *
tail
16 U U "probably a hexamer", interact in crystal [53]
17 V V   [51, 5456]
18 V GT the T domain binds soluble V [24]
19 H G/GT G/GT hold H in an extended fashion [24]
20 H V V probably assembles around H, displacing G/GT [57]
replication
21 O O O-O interactions when bound to ori DNA [58]
22 O P   [5962]
transcription
23 CI CI Forms octamer that links OR to OL [63, 64]
24 CII CII homotetramers [65]
25 CIII CIII dimer [66]
26 Cro Cro dimer; x-ray structure [67]
Recombination
27 Exo Bet   [68]
28 Xis Int   [69] #
29 Xis Xis Xis-Xis binding mediates cooperative DNA-binding [69] #
30 Int Int Dimer [70]
lysis
31 Rz Rz1 heteromultimer that is supposed to span the periplasm [71]
32 S S large ring in inner membrane [72]
  S S' S' inhibits S ring formation (S: 105 aa, S': 107 aa) [73]
lysogenic conversion
33 SieB Esc Esc is encoded in frame in sieB + inhbits sieB [74, 75] #
  1. bold: found in this study. * unpublished (C. Catalano, pers. comm., by permission), # interactions not tested in Y2H assays (one or both clones not available).