Table 2 Bacterial strains and plasmids

536 wt

UPEC wild type strain, Sm^R

[69]

536-21

536, ΔPAI I₅₃₆ ΔPAI II₅₃₆, Sm^R

[2]

536-19/1mob

Donor strain in the mobilisation experiments, pir _λatt , mob_GP704 inserted in PAI II₅₃₆, pRP4, Sm^R, Ap^R, Cm^R, Tc^R, Km^R

This study

SY327λpir

F^-, araD, Δ(lac pro), argE(Am), recA56, Rif^R, gyrA λpir

[60]

SM10λpir

thi1, thr1, leuB6, supE44, tonA21, lacY1, recA::RP4-2-Tc::Mu λpir Km^R

[60]

SY327-23

Mobilised PAI II₅₃₆ is integrated into leuX

This study

SY327-77

Mobilised PAI II₅₃₆ is present as a CI

This study

Plasmids

pGEM^®T-Easy

bla, T/A cloning vector

Promega

pGP704

bla, oriR6K, mobRP4

[60]

pSG704

cat, oriR6K, mobRP4

This study

pCVD442Tc

bla, tet, oriR6K, mobRP4, sacB

This study

pLDR8 neo, int expression vector, Ts

neo, int expression vector, Ts

[62]

pLDR9

bla neo, cloning vector to integrate DNA into attB

[62]

pPAI II-CI

bla, positive control for detection of PAI II₅₃₆-specific CIs

[17]