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Table 1 Mobilisation and remobilisation of PAI II536

From: Mobilisation and remobilisation of a large archetypal pathogenicity island of uropathogenic Escherichia coli in vitrosupport the role of conjugation for horizontal transfer of genomic islands

 

Transfer rate of PAI II536

 

20°C

37°C

Mobilisation rate from E. coli 536 to E. coli SY327

  

Donor-recipient ratio 3:1

3.47 × 10-08 ± 4.85 × 10-09

3.65 × 10-08 ± 5.46 × 10-09

Donor-recipient ratio 9:1

4.93 × 10-08 ± 1.14 × 10-08

4.31 × 10-08 ± 6.11 × 10-09

Remobilisation rate from E. coli SY327 to E. coli 536-21

  

Donor with integrated PAI II536

1.41 × 10-07 ± 1.25 × 10-07

8.00 × 10-08 ± 7.47 × 10-08

Donor with CI of PAI II536

4.32 × 10-05 ± 3.65 × 10-05

3.75 × 10-05 ± 3.18 × 10-05

  1. 31 and 10 independent conjugation experiments were performed for the mobilisation and remobilisation experiment, respectively. Plasmid RP4 was used as a helper plasmid for mobilisation of the excised PAI II536 construct from E. coli 536 into recipient E. coli SY327.
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BMC Microbiology

ISSN: 1471-2180

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