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Table 1 Mobilisation and remobilisation of PAI II536

From: Mobilisation and remobilisation of a large archetypal pathogenicity island of uropathogenic Escherichia coli in vitrosupport the role of conjugation for horizontal transfer of genomic islands

  Transfer rate of PAI II536
  20°C 37°C
Mobilisation rate from E. coli 536 to E. coli SY327   
Donor-recipient ratio 3:1 3.47 × 10-08 ± 4.85 × 10-09 3.65 × 10-08 ± 5.46 × 10-09
Donor-recipient ratio 9:1 4.93 × 10-08 ± 1.14 × 10-08 4.31 × 10-08 ± 6.11 × 10-09
Remobilisation rate from E. coli SY327 to E. coli 536-21   
Donor with integrated PAI II536 1.41 × 10-07 ± 1.25 × 10-07 8.00 × 10-08 ± 7.47 × 10-08
Donor with CI of PAI II536 4.32 × 10-05 ± 3.65 × 10-05 3.75 × 10-05 ± 3.18 × 10-05
  1. 31 and 10 independent conjugation experiments were performed for the mobilisation and remobilisation experiment, respectively. Plasmid RP4 was used as a helper plasmid for mobilisation of the excised PAI II536 construct from E. coli 536 into recipient E. coli SY327.