Analysis of the genomic restriction pattern of different recipient clones upon transfer of PAI II
by PFGE. Genomic DNA of three representatives of the transconjugants carried either a chromosomally inserted PAI II536 or an episomal circular intermediate (CI) were digested with SfiI. Donor strain 536 and recipient strain SY327λpir are controls. Recipients 26, 59, and 77 (marked with 'o') carried a PAI II536-specific CI, whereas in strains 23, 46, and 54 PAI II536 has been chromosomally inserted at the leuX tRNA locus. L, Lambda Ladder PFGE marker, (New England Biolabs).