Confirmation of the chromosomal insertion of the mobilised PAI II
in recipient strain SY327. leuX and PAI II536- specific PCRs were carried out (A) with laboratory K-12 strain SY327λpir, wild type strain 536, and the transconjugant clones 23, 46, 54. For this purpose, four test primers (M803b, M805c, PaiIIrev1, PaiIIfw53) were used in different combinations indicated in (B). The orientation of the primers relative to leuX (grey box) in a K-12 strain and in the wild type strain 536 is depicted in the lower part of the figure (C).