Figure 3

Identification and cladistic analysis of IMPDH-A and IMPDH-B coding genes from different fungi. A) Gene organization of imdA from A. nidulans and mpaF (coding for IMPDH-B in P. brevicompactum). The sequence region used for creating the cladograms in B is marked by a square. Introns are marked by a thin open line. B) and C): Rooted cladograms based on, B) IMPDH cDNA sequences (651-654 bp); and C) β-tubulin cDNA sequences (981 bp) from species from Penicillium subgenus Penicillium and from five fungi with sequenced genomes including the outgroup. P.: Penicillium and A.: Aspergillus. Bootstrap values (expressed as percentage of 1000 replications) are shown at the branch points. MPA production is indicated by "+" or "-". The clades with Penicillium subgenus Penicillium genes are boxed; red, IMPDH-A; blue, IMPDH-B; green, β-tubulin. Coccidioides immitis has been used as outgroup in both analyses B and C. Scale bars correspond to 0.130 and 0.060 nucleotide changes per site in cladograms B) and C) respectively.