Table 2 Oligonucleotide primers for Real-Time PCR detection of genes encoding for resistance to vancomycin (vanA, vanB, vanC1, vanC2), tetracycline (tet(L), tet(M), tet(S)), ciprofloxacin (gyrA), ampicillin (pbp5) and gentamicin (aac(6')-aph(2'))
From: SNP diversity of Enterococcus faecalis and Enterococcus faecium in a South East Queensland waterway, Australia, and associated antibiotic resistance gene profiles
|
Target gene
|
Primer name
|
Primer sequence (5' to3')
|
Positive control
|
|---|
|
van A
|
vanAFa
|
TGTGCGGTATTGGGAAACAG
|
ATCC 51559
|
| |
vanARb
|
GATTCCGTACTGCAGCCTGATT
| |
|
van B
|
vanBF
|
TCTGCTTGTCATGAAAGAAAGAGAA
|
ATCC 700802
|
| |
vanBR
|
GCATTTGCCATGCAAAACC
| |
|
tet(L)
|
tetLF
|
GGGTAAAGCATTTGGTCTTATTGG
|
RBH200523
|
| |
tetLR
|
ATCGCTGGACCGACTCCTT
| |
|
tet(M)
|
tetMF
|
GCAGAATATACCATTCACATCGAAGT
|
RBH200535
|
| |
tetMR
|
AAACCAATGGAAGCCCAGAA
| |
|
tet(S)
|
tetSF
|
CCATTGATATCGAAGTACCTCCAA
|
RBH200535
|
| |
tetSR
|
AGGAAGTGGTGTTACAGATAAACCAA
| |
|
gyr A
|
gyrAF
|
CGGATGAACGAATTGGGTGTGA
|
ATCC 51559
|
| |
gyrAR
|
AATTTTACTCATACGTGCTT
| |
|
pbp 5
|
pbp5F
|
GTTCTGATCGAACATGAAGTTCAAA
|
ATCC 51559
|
| |
pbp5R
|
TGTGCCTTCGGATCGATTG
| |
|
aac(6')-aph(2')
|
acc-aphF
|
TCCTTACTTAATGACCGATGTACTCT
|
ATCC 700802
|
| |
acc-aphR
|
TCTTCGCTTTCGCCACTTTGA
| |
- Fa forward primer, Rb reverse primer
