Table 2 Oligonucleotide primers for Real-Time PCR detection of genes encoding for resistance to vancomycin (vanA, vanB, vanC1, vanC2), tetracycline (tet(L), tet(M), tet(S)), ciprofloxacin (gyrA), ampicillin (pbp5) and gentamicin (aac(6')-aph(2'))
Target gene | Primer name | Primer sequence (5' to3') | Positive control |
|---|---|---|---|
van A | vanAFa | TGTGCGGTATTGGGAAACAG | ATCC 51559 |
| Â | vanARb | GATTCCGTACTGCAGCCTGATT | Â |
van B | vanBF | TCTGCTTGTCATGAAAGAAAGAGAA | ATCC 700802 |
| Â | vanBR | GCATTTGCCATGCAAAACC | Â |
tet(L) | tetLF | GGGTAAAGCATTTGGTCTTATTGG | RBH200523 |
| Â | tetLR | ATCGCTGGACCGACTCCTT | Â |
tet(M) | tetMF | GCAGAATATACCATTCACATCGAAGT | RBH200535 |
| Â | tetMR | AAACCAATGGAAGCCCAGAA | Â |
tet(S) | tetSF | CCATTGATATCGAAGTACCTCCAA | RBH200535 |
| Â | tetSR | AGGAAGTGGTGTTACAGATAAACCAA | Â |
gyr A | gyrAF | CGGATGAACGAATTGGGTGTGA | ATCC 51559 |
| Â | gyrAR | AATTTTACTCATACGTGCTT | Â |
pbp 5 | pbp5F | GTTCTGATCGAACATGAAGTTCAAA | ATCC 51559 |
| Â | pbp5R | TGTGCCTTCGGATCGATTG | Â |
aac(6')-aph(2') | acc-aphF | TCCTTACTTAATGACCGATGTACTCT | ATCC 700802 |
| Â | acc-aphR | TCTTCGCTTTCGCCACTTTGA | Â |