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Table 1 E. faecium strains and plasmids used in this work

From: The hyl Efm gene in pHylEfm of Enterococcus faecium is not required in pathogenesis of murine peritonitis

Strains/Plasmids Relevant Characteristics Reference
Strains   
E. faecium   
TX16 (DO) Sequenced endocarditis clinical isolate, Emr, Smr. ST-16a http://www.hgsc.bcm.tmc.edu [35]
TX1330RF Fsr and Rfr derivative of TX1330, a faecal colonizing strain from a healthy human volunteer [11]
TX1330RF (pHylEfmTX16) Derivative of TX1330RF to which the hyl Efm -containing plasmid (pHylEfmTX16) was transferred by conjugation from TX16 (DO) (~250 kb) [11]
TX1330RF (pHylEfmTX16Δ7,534) Mutant with deletion of part or all of 6 genes of the hyl Efm region of TX1330RF(pHylEfmTX16) This work
TX1330RF (pHylEfmTX16Δ4genes) Non-polar deletion of 4 genes of the hyl Efm region of TX1330RF(pHylEfmTX16) This work
TX1330RF (pHylEfmTX16Δ hyl ) Non-polar deletion mutant of hyl Efm of TX1330RF(pHylEfmTX16) This work
TX1330RF (pHylEfmTX16Δ hyl-down ) Non-polar deletion of hyl Efm plus its downstream gene of TX1330RF(pHylEfmTX16) This work
TX1330RF (pHylEfmTX16Δ down ) Non-polar deletion of the gene downstream of hyl Efm of TX1330RF(pHylEfmTX16) This work
E. faecalis   
CK111 OG1Sp upp4::P23 repA4 [25]
Plasmids   
pHylEfmTX16 Conjugative and transferable megaplasmid (ca. 250 kb) of TX16 (DO) containing hyl Efm [11]
pCJK47 Conjugative donor plasmid for markerless mutagenesis; oriTpCF10 and pheS* pORI280 derivative; confers Emr [25]
pHOU1 Derivative of pCJK47 in which the erm(C) gene was replaced by aph-2'-ID; confers Gmr This work
pHOU2 Derivative of pCJK47 in which the erm(C) gene was replaced by aph-2'-ID and cat was incorporated in the cloning site for allelic replacements; confers Gmr. This work
pTEX5501ts E. coli-enterococcal shuttle plasmid for mutagenesis using a temperature-sensitive replicon [27]
pAT392 oriRpAMβ1, oriRpUC oriTRK2 spc lacZα P2 aac(6')-aph(2") [30]
pAT392::hyl Efm Derivative of pAT392 containing hyl Efm (cloned with SacI and SmaI) under the control of the P2 promoter This work
pAT392::hyl Efm- down Derivative of pAT392 containing both the hyl Efm plus downstream genes (cloned with SacI and SmaI) under the control of the P2 promoter This work
  1. Emr, erythromycin resistance; Fsr, fusidic acid resistance; Gmr, gentamicin resistance, Rfr, rifampin resistance; Smr, high-level resistance to streptomycin. aST refers to sequence type after multi-locus sequence typing. ST16 is part of CC17