Figure 4

Growth and survival curves in the mouse peritonitis model of E. faecium TX0016(pHyl _EfmTX16 ) and TX1330RF(pHyl _EfmTX16 ), carrying an intact hyl _Efm -region, and pHyl _{EfmTX16Δ7,534} (6 gene mutant of the hyl _Efm -region). A, Survival curve of representative inoculum (5 inocula per experiment in two independent experiments) of TX0016(pHyl_EfmTX16) vs TX0016(pHyl_{EfmTX16Δ7,534}) in mouse peritonitis; B, growth curves of TX1330RF(pHyl_EfmTX16) vs TX1330RF(pHyl_{EfmTX16Δ7,534}) and a second transconjugant [TX1330RF(pHyl_{EfmTX16Δ7,534})-TCII] obtained from the same mating experiment between TX16(pHyl_{EfmTX16Δ7,534}) and TX1330RF, expressed as optical density (A₆₀₀) in brain heart infusion (BHI) broth (results of at least three experiments per strain). C and D, survival curves of TX1330RF(pHyl_EfmTX16) vs TX1330RF(pHyl_{EfmTX16Δ7,534}) obtained in the peritonitis model at different inocula in independent experiments performed at different days.