Figure 3

Transcriptional analysis of genes in the hyl _Efm region using reverse transcriptase (RT)-PCR. A, physical map of the hyl _Efm region and primers used for RT-PCR experiments_. Black arrows above the genes indicate the position of the primers used to amplify DNA sequences from the cDNA obtained after reverse transcription. B, RT-PCR using primers A1-A2; C, RT-PCR using primes B1-B2; D, RT-PCR using primers C1-C2; E, RT-PCR using primers D1-D2; F, RT-PCR with ddl as the target gene using primers E1-E2 (Table 2) [32, 33]. Lanes 1 and 2, TX1330RF (RT-PCR reaction and control without RT enzyme, respectively); lanes 3 and 4, TX1330RF(pHyl_Efm16) (RT-PCR reaction and control without RT enzyme, respectively); lanes 5 and 6 TX16(pHyl_Efm16) (RT-PCR reaction and control without RT enzyme respectively). The molecular weight of the bands is indicated to the right.