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Table 6 Strains and plasmids used in this study

From: Iron restriction induces preferential down-regulation of H2-consuming over H2-evolving reactions during fermentative growth of Escherichia coli

Strains/plasmids Genotype Reference
MC4100 F- araD139 Δ(argF-lac)U169 ptsF25 deoC1 relA1 flbB5301 rspL150 - [37]
DHP-F2 MC4100 ΔhypF 59-629AA [16]
XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F' proAB lacIqZΔM15 Tn10 (TetR)] Stratagene
PM06 Like MC4100 but feoB::Tn5 This study
PX06 Like XL1-Blue but feoB::Tn5 This study
CP411 Like MC4100 but ΔentC::cat feoB::Tn5 This study
CP413 Like MC4100 but ΔfecA-E ΔentC::cat feoB::Tn5 This study
CP415 Like MC4100 but ΔfecA-E ΔentC::cat This study
CP416a Like MC4100 but ΔentC::cat This study
CP422 Like MC4100 but ΔfecA-E introduced from GG7 This study
GG7 W3110 ΔfecA-E::kan G. Grass
CP971 MC4100 ΔhycAI::kan [38]
CP612 Like MC4100 but Φ(hyaA'-'lacZ) This study
CP775 Like MC4100 but Φ(hybO'-'lacZ) This study
CP951 Like MC4100 but Φ(hycA'-'lacZ) This study
CP1069 Like MC4100 but ΔhypF Φ(hyaA'-'lacZ) This study
CP1084 Like MC4100 but ΔhypF Φ(hybO'-'lacZ) This study
CP1149 Like MC4100 but ΔhypF Φ(hycA'-'lacZ) This study
CP1073 Like MC4100 but ΔfecA-E Φ(hyaA'-'lacZ) This study
CP1088 Like MC4100 but ΔfecA-E Φ(hybO'-'lacZ) This study
CP1150 Like MC4100 but ΔfecA-E Φ(hycA'-'lacZ) This study
CP1075 Like MC4100 but ΔfeoBb Φ(hyaA'-'lacZ) This study
CP1090 Like MC4100 but ΔfeoBb Φ(hybO'-'lacZ) This study
CP1151 Like MC4100 but ΔfeoBb Φ(hycA'-'lacZ) This study
CP1071 Like MC4100 but ΔentC Φ(hyaA'-'lacZ) This study
CP1086 Like MC4100 but ΔentC Φ(hybO'-'lacZ) This study
CP1152 Like MC4100 but ΔentC Φ(hycA'-'lacZ) This study
CP1079 Like MC4100 but ΔfecA-E feoBb Φ(hyaA'-'lacZ) This study
CP1094 Like MC4100 but ΔfecA-E feoBb Φ(hybO'-'lacZ) This study
CP1153 Like MC4100 but ΔfecA-E feoBb Φ(hycA'-'lacZ) This study
CP1081 Like MC4100 but ΔentC feoBb Φ(hyaA'-'lacZ) This study
CP1096 Like MC4100 but ΔentC feoBb Φ(hybO'-'lacZ) This study
CP1154 Like MC4100 but ΔentC feoBb Φ(hycA'-'lacZ) This study
CP1077 Like MC4100 but ΔentC fecA-E Φ(hyaA'-'lacZ) This study
CP1092 Like MC4100 but ΔentC fecA-E Φ(hybO'-'lacZ) This study
CP1155 Like MC4100 but ΔentC fecA-E Φ(hycA'-'lacZ) This study
CP1083 Like MC4100 but ΔentC fecA-E feoBb Φ(hyaA'-'lacZ) This study
CP1098 Like MC4100 but ΔentC fecA-E feoBb Φ(hybO'-'lacZ) This study
CP1163 Like MC4100 but ΔentC fecA-E feoBb Φ(hycA'-'lacZ) This study
Plasmids   
pFEO feoABC+ from E. coli in pASK-IBA7 [39]
pECD 1079 feoB+ from E. coli in pASK-IBA7 N. Taudte and G. Grass
pRS552 KmR ApR lacZ+ lacY+ lacA+ [20]
phyaA552 like pRS552 but containing Φ(hyaA'-'lacZ) This study
phybO552 like pRS552 but containing Φ(hybO'-'lacZ) This study
pTL101 like pRS552 but containing Φ(hycA'-'lacZ), cloned from PstI within hycA to AvaII within hycA [28]
  1. a P1 lysate from ΔentC::cat was obtained from G. Grass and N. Taudte
  2. b Similar to PM06, these strains were constructed using P1kc lysate grown on the ΔfeoB::kan knockout mutant JW3372 from the Keio collection [40] with subsequent elimination of the kanamycin resistance cassette [41].