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Table 5 Influence of iron transport mutations on expression of hyaA, hybO and hycA lacZ fusions

From: Iron restriction induces preferential down-regulation of H2-consuming over H2-evolving reactions during fermentative growth of Escherichia coli

 

β-Galactosidase specific activity in Miller Units (± standard deviation)

Strain/genotypea

Φ(hyaA'-'lacZ)

Φ(hybO'-'lacZ)

Φ(hycA'-'lacZ)

MC4100 (wild type)

818 ± 232

52 ± 46

44 ± 9

MC4100 aerobically

12 ± 3

12 ± 3

13 ± 2

MC4100 + 15 mM formate

770 ± 535

87 ± 30

126 ± 57

DHP-F2 (ΔhypF)

620 ± 221

60 ± 27

53 ± 22

ΔfecA-E

633 ± 252

52 ± 17

41 ± 11

ΔfeoB

355 ± 96

36 ± 7

65 ± 40

ΔentC

410 ± 110

40 ± 15

33 ± 20

ΔfecA-E feoB

491 ± 139

43 ± 11

28 ± 13

ΔentC fecA-E feoB

371 ± 94

45 ± 11

35 ± 24

ΔentC feoB

574 ± 155

45 ± 21

49 ± 32

ΔentC fecA-E

340 ± 211

47 ± 12

57 ± 19

  1. a In the interest of clarity only the genotype of the strains is given. The strains used can be found in Table 6. Growth was performed under fermentative conditions in TGYEP, unless indicated otherwise.
  2. n. d.-not determined
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BMC Microbiology

ISSN: 1471-2180

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