Figure 3
From: Lysis-deficient phages as novel therapeutic agents for controlling bacterial infection
Complementation with heterologous endolysin gene for enrichment of endolysin-deficient phage P954. Ten-fold serial dilutions of endolysin-deficient phage P954 (5 × 1010 PFU/ml) spotted on (a) S. aureus RN4220 lawn and (b) complementing host pGMB540/S. aureus RN4220, which expresses a heterologous endolysin. Plaque assay of enriched endolysin-deficient phage P954 on (c) non-complementing host S. aureus RN4220 and (d) complementing host pGMB540/S. aureus RN4220.