Genotypic and phenotypic diversity of Ralstonia pickettii and Ralstonia insidiosa isolates from clinical and environmental sources including High-purity Water. Diversity in Ralstonia pickettii

Table 2 Oligonucleotides used in this study

Primer	Oligonucleotide Sequence 5'-3'	Target^a	Product size	Reference
Rp-F1	ATGATCTAGCTTGCTAGATTGAT	16S rRNA gene	210 bp	[32]
Rp-R1	ACTGATCGTCGCCTTGGTG	16S rRNA gene		[32]
R38R1	CACACCTAATATTAGTAAGTGCG	16S rRNA gene	403 bp	[33]
16SF	TTGTACACACCGCCCGTCA	16S-23S Spacer Region	860 bp	[34]
23SR	GGTACCTTAGATGTTTCAGTTC	16S-23S Spacer Region		[34]
Ral_fliCF	CCTCAGCCTCAATACCAACATC	fliC gene	725 bp	[35]
Ral_fliCR	CATGTTCGACGTTTCMGAWGC	fliC gene		[35]
M13	TTATGTAAAACGACGGCCAGT	RAPD Primer	N\A	[36]
P3	AGACGTCCAC	RAPD Primer	N\A	[37]
P15	AATGGCGCAG	RAPD Primer	N\A	[37]
OPA03U	AGTCAGCCAC	RAPD Primer	N\A	[38]
BOX-A1R	CTACGGCAAGGCGACGCTGACG	BOX Primer	N\A	[39]

ISSN: 1471-2180