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Table 1 Microorganisms evaluated for susceptibility-resistance to antibiotics that inhibit peptidoglycan synthesis

From: A rapid in situ procedure for determination of bacterial susceptibility or resistance to antibiotics that inhibit peptidoglycan biosynthesis

Gram

Bacteria

Antibiotics- CLSI MIC Interpretative Standard (μg/mL)

CLSI Category

MIC (μg/ml)

Drug concentration at which the nucleoids were spread - and DNA fragments were released

Gram -

Acinetobacter baumannii

Imipenem: ≤ 4 - 8 - ≥16 (SI, R)

Susceptible

2

4-4

Gram -

Acinetobacter baumannii

Imipenem: ≤ 4 - 8 - ≥16 (SI, R)

Intermediate

8

16-16

Gram -

Acinetobacter baumannii

Imipenem: ≤ 4 - 8 - ≥16 (SI, R)

Resistant

> 16

No nucleoids-No fragments

Gram -

Acinetobacter baumannii

Imipenem: ≤ 4 - 8 - ≥16 (SI, R)

Resistant

> 16

No nucleoids-No fragments

Gram -

Acinetobacter baumannii

Ceftazidime: ≤ 8 - 16 - ≥32 (S, I, R)

Susceptible

4

8-8

Gram -

Acinetobacter baumannii

Ceftazidime: ≤ 8 - 16 - ≥32 (S, I, R)

Intermediate

12

32-32

Gram -

Acinetobacter baumannii

Ceftazidime: ≤ 8 - 16 - ≥32 (S, I, R)

Resistant

> 256

No nucleoids-No fragments

Gram -

Enterobacter cloacae

Imipenem: ≤ 1 - 2 - ≥4 (S, I, R)

Susceptible

< 1

1-1

Gram -

Enterobacter cloacae

Imipenem: ≤ 1 - 2 - ≥4 (S, I, R)

Susceptible

< 1

1-1

Gram -

Enterobacter cloacae

Ceftazidime: ≤ 4 - 8 - ≥16 (S, I, R)

Susceptible

< 1

4-4

Gram -

Enterobacter cloacae

Ceftazidime: ≤ 4 - 8 - ≥16 (S, I, R)

Susceptible

< 1

4-4

Gram -

Escherichia coli

Ampicillin: ≤ 8 - 16- ≥32 (S, I, R)

Susceptible

2

8-8

Gram -

Escherichia coli

Ampicillin: ≤ 8 - 16- ≥32 (S, I, R)

Intermediate

12

16-16

Gram -

Escherichia coli

Ampicillin: ≤ 8 - 16- ≥32 (S, I, R)

Resistant

256

No nucleoids-No fragments

Gram -

Escherichia coli

Ceftazidime: ≤ 4 -8- ≥16 (S, I, R)

Susceptible

0.25

4-4

Gram -

Escherichia coli

Ceftazidime: ≤ 4 -8- ≥16 (S, I, R)

Resistant

32

No nucleoids-No fragments

Gram -

Klebsiella oxytoca

Imipenem: ≤ 1 - 2 - ≥4 (S, I, R)

Susceptible

< 1

1-1

Gram -

Klebsiella oxytoca

Ceftazidime: ≤ 4 - 8 - ≥16 (S, I, R)

Susceptible

< 1

4-4

Gram -

Klebsiella spp.

Imipenem: ≤ 1 - 2 - ≥4 (S, I, R)

Susceptible

< 1

1-1

Gram -

Klebsiella spp.

Imipenem: ≤ 1 - 2 - ≥4 (S, I, R)

Susceptible

< 1

1-1

Gram -

Klebsiella spp.

Imipenem: ≤ 1 - 2 - ≥4 (S, I, R)

Susceptible

< 1

1-1

Gram -

Klebsiella spp.

Ceftazidime: ≤ 4 - 8 - ≥16 (S, I, R)

Intermediate

8

16-16

Gram -

Klebsiella spp.

Ceftazidime: ≤ 4 - 8 - ≥16 (S, I, R)

Resistant

> 16

No nucleoids-No fragments

Gram -

Klebsiella spp.

Ceftazidime: ≤ 4 - 8 - ≥16 (S, I, R)

Resistant

> 16

No nucleoids-No fragments

Gram -

Morganella morganii

Imipenem: ≤ 1 - 2 -≥4 (S, I, R)

Intermediate

2

4-4

Gram -

Morganella morganii

Ceftazidime: ≤ 4 - 8 -≥16 (S, I, R)

Susceptible

< 1

4-4

Gram -

Proteus mirabilis

Imipenem: ≤ 1 - 2 - ≥4 (S, I, R)

Intermediate

2

4-4

Gram -

Proteus mirabilis

Ceftazidime: ≤ 4 - 8 - ≥16 (S, I, R)

Susceptible

< 1

4-4

Gram -

Salmonella spp

Imipenem: ≤ 1 - 2 - ≥4 (S, I, R)

Susceptible

< 1

1-1

Gram -

Salmonella spp

Ceftazidime: ≤ 4 - 8 - ≥16 (S, I, R)

Susceptible

< 1

4-4

Gram+

Enterococcus faecalis

Ampicillin: ≤ 8 - ≥16 (S, R)

Susceptible

4

8-No fragments

Gram+

Enterococcus faecalis

Penicillin: ≤ 8 - ≥16 (S, R)

Susceptible

2

8-No fragments

Gram+

Enterococcus faecalis

Vancomycin: ≤ 4 -8/16- ≥32 (S, I, R)

Resistant

> 32

No nucleoids-No fragments

Gram+

Enterococcus faecium

Ampicillin: ≤ 8 - ≥16 (S, R)

Resistant

> 32

No nucleoids-No fragments

Gram+

Enterococcus faecium

Penicillin: ≤ 8 - ≥16 (S, R)

Resistant

> 32

No nucleoids-No fragments

Gram+

Enterococcus faecium

Vancomycin: ≤ 4 -8/16- ≥32 (S, I, R)

Susceptible

< 1

4-No fragments

Gram+

Enterococcus spp.

Ampicillin: ≤ 8 - ≥16 (S, R)

Susceptible

< 1

8-No fragments

Gram+

Enterococcus spp.

Ampicillin: ≤ 8 - ≥16 (S, R)

Intermediate

12

16-No fragments

Gram+

Enterococcus spp.

Ampicillin: ≤ 8 - ≥16 (S, R)

Resistant

> 16

No nucleoids-No fragments

Gram+

Enterococcus spp.

Penicillin: ≤ 8 - ≥16 (S, R)

Susceptible

2

8-No fragments

Gram+

Enterococcus spp.

Penicillin: ≤ 8 - ≥16 (S, R)

Resistant

> 16

No nucleoids-No fragments

Gram+

Enterococcus spp.

Vancomycin: ≤ 4 -8/16- ≥32 (S, I, R)

Susceptible

2

4-No fragments

Gram+

Enterococcus spp.

Vancomycin: ≤ 4 -8/16- ≥32 (S, I, R)

Resistant

> 32

No nucleoids-No fragments

Gram+

Enterococcus spp.

Vancomycin: ≤ 4 -8/16- ≥32 (S, I, R)

Resistant

> 32

No nucleoids-No fragments

Gram+

Staphylococcus aureus

Vancomycin: ≤2 - 4-8 - ≥16 (S, I, R)

Susceptible

< 1

2-No fragments

Gram+

Staphylococcus aureus

Vancomycin: ≤2 - 4-8 - ≥16 (S, I, R)

Susceptible

< 1

2-No fragments