|
Primer
|
Gene
|
Direction
|
Sequence (5' to 3')
|
Location
|
|---|
|
mactF-RT
|
act
|
F
|
CCGCCCTCGTGATTGATAAC
|
Spanning exons 2 & 3
|
|
mactR-RT
|
act
|
R
|
TCACCAACGTAGGAGTCCTT
|
Spanning exons 4 & 5
|
|
mmcrtYBF2-RT
|
crtYB(mm)
|
F
|
TCGCATATTACCAGATCCATCTGA
|
Spanning exons 1 & 2
|
|
mmcrtYBR2-RT
|
crtYB(mm)
|
R
|
GGATATGTCCATGCGCCATT
|
Exon 2
|
|
amcrtYBF-RT
|
crtYB(am)
|
F
|
GTGTGCATATGTGTTGCAACCA
|
Spanning exon 1 & intron 2
|
|
amcrtYBR-RT
|
crtYB(am)
|
R
|
AGAAGGTGCCTAGTTGCCAAGA
|
Exon 3
|
|
mmcrtIF-RT
|
crtI(mm)
|
F
|
CATCGTGGGATGTGGTATCG
|
Spanning exons 1 & 2
|
|
mmcrtIR-RT
|
crtI(mm)
|
R
|
GGCCCCTGATCGAATCGATAA
|
Spanning exons 3, 4, 5
|
|
amcrtIF-RT
|
crtI(am)
|
F
|
CGTGGTTTAATCCGTATCAGC
|
Spanning exon 1 & intron 1
|
|
amcrtIR2-RT
|
crtI(am)
|
R
|
TCTCGAACACCGTGACCT
|
Exon 2
|
|
mcrtSF-RT
|
crtS
|
F
|
ATGGCTCTTGCAGGGTTTGA
|
Spanning exons 6 & 7
|
|
mcrtSR-RT
|
crtS
|
R
|
TGCTCCATAAGCTCGATCCCAA
|
Spanning exons 8 & 9
|
|
grg2real FW1
|
grg2
|
F
|
CATCAAGACCTCTGTCACCAAC
|
Spanning exons 1 & 2
|
|
grg2real RV1
|
grg2
|
R
|
TTGGCGTCAGACGAGGACT
|
Exon 3
|
|
pdcreal FW1
|
PDC
|
F
|
TCAACACTGAGCTGCCCACT
|
Spanning exons 5 & 6
|
|
pdcreal RV1
|
PDC
|
R
|
ATTCCGAATCGGGAAGCACA
|
Exon 6
|
- F: Forward, R: Reverse; (mm): mature transcript, (am): alternatively spliced transcript.
