Table 1 Primers used in this work
Primer | Gene | Direction | Sequence (5' to 3') | Location |
|---|---|---|---|---|
mactF-RT | act | F | CCGCCCTCGTGATTGATAAC | Spanning exons 2 & 3 |
mactR-RT | act | R | TCACCAACGTAGGAGTCCTT | Spanning exons 4 & 5 |
mmcrtYBF2-RT | crtYB(mm) | F | TCGCATATTACCAGATCCATCTGA | Spanning exons 1 & 2 |
mmcrtYBR2-RT | crtYB(mm) | R | GGATATGTCCATGCGCCATT | Exon 2 |
amcrtYBF-RT | crtYB(am) | F | GTGTGCATATGTGTTGCAACCA | Spanning exon 1 & intron 2 |
amcrtYBR-RT | crtYB(am) | R | AGAAGGTGCCTAGTTGCCAAGA | Exon 3 |
mmcrtIF-RT | crtI(mm) | F | CATCGTGGGATGTGGTATCG | Spanning exons 1 & 2 |
mmcrtIR-RT | crtI(mm) | R | GGCCCCTGATCGAATCGATAA | Spanning exons 3, 4, 5 |
amcrtIF-RT | crtI(am) | F | CGTGGTTTAATCCGTATCAGC | Spanning exon 1 & intron 1 |
amcrtIR2-RT | crtI(am) | R | TCTCGAACACCGTGACCT | Exon 2 |
mcrtSF-RT | crtS | F | ATGGCTCTTGCAGGGTTTGA | Spanning exons 6 & 7 |
mcrtSR-RT | crtS | R | TGCTCCATAAGCTCGATCCCAA | Spanning exons 8 & 9 |
grg2real FW1 | grg2 | F | CATCAAGACCTCTGTCACCAAC | Spanning exons 1 & 2 |
grg2real RV1 | grg2 | R | TTGGCGTCAGACGAGGACT | Exon 3 |
pdcreal FW1 | PDC | F | TCAACACTGAGCTGCCCACT | Spanning exons 5 & 6 |
pdcreal RV1 | PDC | R | ATTCCGAATCGGGAAGCACA | Exon 6 |