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Table 2 Oligonucleotide primers used for the construction of chimeras and mutants of PCV2

From: Identification of one critical amino acid that determines a conformational neutralizing epitope in the capsid protein of porcine circovirus type 2

Chimera/mutant

Primer

Sequence

rCL-YJ-1

A-F

5'-AGATTTAATATTAACGACTTTGTTC-3'

 

A-R

5'-GTTGAAGATGCCATTTTTCCT-3'

 

B-F

5'-AGGAAAAATGGCATCTTCAACGCCCGCCTCTCCCGCACCTTCGGATATACTATCAAGCGAG

CCACAGTCAAAACGCCCTCCTGGGCGGTGGACATGATGAGATTTAATATTAACGACTTT-3'

 

B-R

5'-AAAGTCGTTAATATTAAATCTCATCATGTCCACCGCCCAGGAGGGCGTTTTGACTGTGGCTC

GCTTGATAGTATATCCGAAGGTGCGGGAGAGGCGGGCGTTGAAGATGCCATTTTTCCT-3'

rCL-YJ-2

C-F

5'-TACTACAGAATAAGGAAGGTTAAGG-3'

 

C-R

5'-AAAGTCGTTAATATTAAATCTAATC-3'

 

D-F

5'-ATTAGATTTAATATTAACGACTTTCTTCCCCCAGGAGGGGGCTCAAACCCCCGCTCTGTGCC

CTTTGAATACTACAGAATAAGGAAGGTTAAG-3'

 

D-R

5'-CTTAACCTTCCTTATTCTGTAGTATTCAAAGGGCACAGAGCGGGGGTTTGAGCCCCCTCCT

GGGGGAAGAAAGTCGTTAATATTAAATCTAAT- 3'

rCL-YJ-3

E-F

5'-TCCTACCACTCCCGGTACTTTA-3'

 

E-R

5'-GGAGCAGGGCCAGAATTCAA-3'

 

F-F

5'-TAAGGTTGAATTCTGGCCCTGCTCCCCGATCACCCAGGGTGACAGGGGAGTGGGCTCCAG

TGCTGCTATTCTAGATGATAACTTTGTAACAAAGGCCACAGCCCTCACCTATGACCCCTATGTA

AACTACTCCTCCCGCCATACCATAACCCAGCCCTTCTCCTACCACTCCCGCTACTTTACCC-3'

 

F-R

5'-GGGTAAAGTACCGGGAGTGGTAGGAGAAGGGCTGGGTTATGGTATGGCGGGAGGAGTAGT

TTACATAGGGGTCATAGGTGAGGGCTGTGGCCTTTGTTACAAAGTTATCATCTAGAATAGCAGC

ACTGGAGC CCACTCCCCTGTCACCCTGGGTGATCGGGGAGCAGGGCCAGAATTCAACCTTA-3'

rCL-YJ-4

G-F

5'-AATATCCGGGTAACTATGTATGT-3'

 

G-R

5'-GGTTTGTAGTCTCAGCCAAAG-3'

 

H-F

5'-CTTTGGCTGAGACTACAAACCGCTGGAAATGTAGACCACGTAGGCCTCGGCACTGCGTTC

GAAAACAGTATATACGACCAGGAATACAATATCCGGGTAACTATGTAT-3'

 

H-R

5'-ATACATAGTTACCCGGATATTGTATTCCTGGTCGTATATACTGTTTTCGAACGCAGTGCCGA

GGCCTACGTGGTCTACATTTCCAGCGGTTTGTAGTCTCAGCCAAAG-3'

rCL-YJ-5

I-F

5'-GACCCCCCACTTAACCTTAATGAATAATAAAAAC-3'

 

I-R

5'-GTTTTTATTATTCATTAAGGTTAAGTGGGGGGTC-3'

rCL-YJ-1-51

J-F

5'-CACCCGCCTCTCCCGCACCTTCGGATA-3'

 

J-R

5'-TATCCGAAGGTGCGGGAGAGGCGGGTG-3'

rCL-YJ-1-57

K-F

5'-ACCTTCGGATATACTATCAAGGCTACCAC-3'

 

K-R

5'-GTGGTAGCCTTGATAGTATATCCGAAGGT-3'

rCL-YJ-1-59

L-F

5'-GGATATACTGTCAAGCGAACCACAGTCAGAAC-3'

 

L-R

5'-GTTCTGACTGTGGTTCGCTTGACAGTATATCC-3'

rCL-YJ-1-63

M-F

5'-GCTACCACAGTCAAAACGCCCTCCTGG-3'

 

M-R

5'-CCAGGAGGGCGTTTTGACTGTGGTAGC-3'

rLG-YJ-1-59

N-F

5'-GGATATACTGTCAAGCGAACCACAGTCAGAAC-3'

 

N-R

5'-GTTCTGACTGTGGTTCGCTTGACAGTATATCC-3'

rJF2-YJ-1-59

O-F

5'-GGATATACTGTCAAGCGAACCACAGTCAGAAC-3'

 

O-R

5'-GTTCTGACTGTGGTTCGCTTGACAGTATATCC-3'

rYJ-CL-1-59

P-F

5'-CGGATATACTATCAAGGCTGCCACAGTCAAAAC-3'

 

P-R

5'-GTTTTGACTGTGGCAGCCTTGATAGTATATCCG-3'

complete genome of PCV2

Q-F

5'-GTCGACGGAGGAAGGGGGCCAGTT-3'

 

Q-R

5'-GTCGACTGTTCTGTAGCATTCTTCCA-3'

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BMC Microbiology

ISSN: 1471-2180

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