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Table 1 Comparisons of T-RFLP profiles of microbiota in the ileum and caecum of layers housed in different cage systems

From: The influence of the cage system and colonisation of SalmonellaEnteritidis on the microbial gut flora of laying hens studied by T-RFLP and 454 pyrosequencing

Before Inoculation

    

Mean SD

Location

Cage

n

T-RF

Conventional

Furnished

Aviary

Ileum

Conventional

4

10.5 ± 1.7

70.5 ± 12.4

-

-

 

Furnished

4

6.5 ± 2.7

67.4 ± 9.9

65.9 ± 7.5

-

 

Aviary

4

7.3 ± 3.5

54.3 ± 9.6

66.8 ± 11.4

72.3 ± 7.0

Caecum

Conventional

4

39.5 ± 6.6

66.4 ± 6.0

-

-

 

Furnished

4

39.8 ± 4.2

60.8 ± 3.5

75.1 ± 6.0

-

 

Aviary

4

52.7 ± 23.5

38.6 ± 6.3

38.5 ± 4.8

45.4 ± 14.5

Four weeks PI

    

Mean SD

Location

Cage

n

T-RF

Conventional

Furnished

Aviary

Ileum

Conventional

8

10.0 ± 1.2

86.5 ± 10.1

-

-

 

Furnished

8

6.9 ± 2.2

65.5 ± 9.3

81.1 ± 6.9

-

 

Aviary

7

10.7 ± 2.7

66.8 ± 9.2

67.5 ± 9.2

73.8 ± 9.0

Caecum

Conventional

8

58.0 ± 5.2

73.4 ± 5.8

-

-

 

Furnished

8

51.3 ± 7.3

57.7 ± 8.1

67.1 ± 8.6

-

 

Aviary

8

63.6 ± 5.3

54.6 ± 4.7

58.2 ± 4.9

74.2 ± 4.9

  1. n: number of samples
  2. SD: Dice similarity coefficient
  3. T-RF: Terminal Restriction Fragments