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Table 1 Primer used for the generation of OppA mutants

From: In Mycoplasma hoministhe OppA-mediated cytoadhesion depends on its ATPase activity

oppA
clone
deletion/mutation (AA) name primer sequence (5'-3') annealing (°C)
ΔCS1 Δ176-243 OppA start 5'-GTGGCGGCCGCGCCTGCAGTTTTTTAG-3' 60°C
   CS1 down 5'-TCTTGATTCAACGTTCTTGTCACCT-3' 60°C
   CS1 up 5'- AAGAACGTTGAATCAAGAGAACTAGATGAAGC-3' 62°C
   OppA end 5'-GGTCCATGGTGGGTACCAAAATAGACCCGGCATATGTAAAA-3' 62°C
ΔCS2 Δ365-372 OppA start 5'-GTGGCGGCCGCGCCTGCAGTTTTTTAG-3' 50°C
   CS2 down 5'-TGAGACGTCTGTAAGCTATCTTTATCCATTGAA-3' 50°C
   CS2 up 5'-AAAGATAGCTTACAATACGCTAAATCTACATTG-3' 62°C
   OppA end 5'-GGTCCATGGTGGGTACCAAAATAGACCCGGCATATGTAAAA-3' 62°C
ΔDC10 Δ366-381 OppA start 5'-GTGGCGGCCGCGCCTGCAGTTTTTTAG-3' 58°C
   DC10 down 5'-CTGACCAATTTTGTATTGTAAGCTATCT-3' 58°C
   DC10 up 5'-TACAAAATTGGTCAGAAAGGTATAGAAAAC-3' 58°C
   OppA end 5'-GGTCCATGGTGGGTACCAAAATAGACCCGGCATATGTAAAA-3' 58°C
ΔCS3 Δ647-675 OppA start 5'-GTGGCGGCCGCGCCTGCAGTTTTTTAG-3' 61°C
   CS3 down 5'-GTACAGCTGTGGAGCATTTAAATATCT-3' 61°C
   CS3 up 5'-GCTCCACAGCTGTACGATCCAAACTTCAA-3' 60°C
   OppA end 5'-GGTCCATGGTGGGTACCAAAATAGACCCGGCATATGTAAAA-3 60°C
ΔWB Δ712-727 OppA start 5'-GTGGCGGCCGCGCCTGCAGTTTTTTAG-3' 50°C
   DC10 down 5'-ATATGCGTTGAAGTTTGGAT-3' 50°C
   DC10 up 5'-TATAACGGTGTTGCTAGCACATAC-3' 58°C
   OppA end 5'-GGGTCCATGGTGGGTACCAAAATAGACCCGGCATATGTAAAA-3' 58°C
WA3 874GKDSSGKS-GLQSYGKT881 OppA start 5'-GTGGCGGCCGCGCCTGCAGTTTTTTAG-3' 60°C
   DC10 down 5'-TACAGATCTGTTGGTTCTATAGTTTTTCCATAACTCTGCAATCCAAAATC-3' 60°C
   DC10 up 5'-CAACAGATCTGTATCAGTGGTCTGCAAT-3' 60°C
   OppA end 5'-GGGTCCATGGTGGGTACCAAAATAGACCCGGCATATGTAAAA-3' 60°C