From: In Mycoplasma hoministhe OppA-mediated cytoadhesion depends on its ATPase activity
oppA clone | deletion/mutation (AA) | name | primer sequence (5'-3') | annealing (°C) |
---|---|---|---|---|
ΔCS1 | Δ176-243 | OppA start | 5'-GTGGCGGCCGCGCCTGCAGTTTTTTAG-3' | 60°C |
 |  | CS1 down | 5'-TCTTGATTCAACGTTCTTGTCACCT-3' | 60°C |
 |  | CS1 up | 5'- AAGAACGTTGAATCAAGAGAACTAGATGAAGC-3' | 62°C |
 |  | OppA end | 5'-GGTCCATGGTGGGTACCAAAATAGACCCGGCATATGTAAAA-3' | 62°C |
ΔCS2 | Δ365-372 | OppA start | 5'-GTGGCGGCCGCGCCTGCAGTTTTTTAG-3' | 50°C |
 |  | CS2 down | 5'-TGAGACGTCTGTAAGCTATCTTTATCCATTGAA-3' | 50°C |
 |  | CS2 up | 5'-AAAGATAGCTTACAATACGCTAAATCTACATTG-3' | 62°C |
 |  | OppA end | 5'-GGTCCATGGTGGGTACCAAAATAGACCCGGCATATGTAAAA-3' | 62°C |
ΔDC10 | Δ366-381 | OppA start | 5'-GTGGCGGCCGCGCCTGCAGTTTTTTAG-3' | 58°C |
 |  | DC10 down | 5'-CTGACCAATTTTGTATTGTAAGCTATCT-3' | 58°C |
 |  | DC10 up | 5'-TACAAAATTGGTCAGAAAGGTATAGAAAAC-3' | 58°C |
 |  | OppA end | 5'-GGTCCATGGTGGGTACCAAAATAGACCCGGCATATGTAAAA-3' | 58°C |
ΔCS3 | Δ647-675 | OppA start | 5'-GTGGCGGCCGCGCCTGCAGTTTTTTAG-3' | 61°C |
 |  | CS3 down | 5'-GTACAGCTGTGGAGCATTTAAATATCT-3' | 61°C |
 |  | CS3 up | 5'-GCTCCACAGCTGTACGATCCAAACTTCAA-3' | 60°C |
 |  | OppA end | 5'-GGTCCATGGTGGGTACCAAAATAGACCCGGCATATGTAAAA-3 | 60°C |
ΔWB | Δ712-727 | OppA start | 5'-GTGGCGGCCGCGCCTGCAGTTTTTTAG-3' | 50°C |
 |  | DC10 down | 5'-ATATGCGTTGAAGTTTGGAT-3' | 50°C |
 |  | DC10 up | 5'-TATAACGGTGTTGCTAGCACATAC-3' | 58°C |
 |  | OppA end | 5'-GGGTCCATGGTGGGTACCAAAATAGACCCGGCATATGTAAAA-3' | 58°C |
WA3 | 874GKDSSGKS-GLQSYGKT881 | OppA start | 5'-GTGGCGGCCGCGCCTGCAGTTTTTTAG-3' | 60°C |
 |  | DC10 down | 5'-TACAGATCTGTTGGTTCTATAGTTTTTCCATAACTCTGCAATCCAAAATC-3' | 60°C |
 |  | DC10 up | 5'-CAACAGATCTGTATCAGTGGTCTGCAAT-3' | 60°C |
 |  | OppA end | 5'-GGGTCCATGGTGGGTACCAAAATAGACCCGGCATATGTAAAA-3' | 60°C |