Skip to main content

Table 1 Primer used for the generation of OppA mutants

From: In Mycoplasma hoministhe OppA-mediated cytoadhesion depends on its ATPase activity

oppA

clone

deletion/mutation (AA)

name

primer sequence (5'-3')

annealing (°C)

ΔCS1

Δ176-243

OppA start

5'-GTGGCGGCCGCGCCTGCAGTTTTTTAG-3'

60°C

  

CS1 down

5'-TCTTGATTCAACGTTCTTGTCACCT-3'

60°C

  

CS1 up

5'- AAGAACGTTGAATCAAGAGAACTAGATGAAGC-3'

62°C

  

OppA end

5'-GGTCCATGGTGGGTACCAAAATAGACCCGGCATATGTAAAA-3'

62°C

ΔCS2

Δ365-372

OppA start

5'-GTGGCGGCCGCGCCTGCAGTTTTTTAG-3'

50°C

  

CS2 down

5'-TGAGACGTCTGTAAGCTATCTTTATCCATTGAA-3'

50°C

  

CS2 up

5'-AAAGATAGCTTACAATACGCTAAATCTACATTG-3'

62°C

  

OppA end

5'-GGTCCATGGTGGGTACCAAAATAGACCCGGCATATGTAAAA-3'

62°C

ΔDC10

Δ366-381

OppA start

5'-GTGGCGGCCGCGCCTGCAGTTTTTTAG-3'

58°C

  

DC10 down

5'-CTGACCAATTTTGTATTGTAAGCTATCT-3'

58°C

  

DC10 up

5'-TACAAAATTGGTCAGAAAGGTATAGAAAAC-3'

58°C

  

OppA end

5'-GGTCCATGGTGGGTACCAAAATAGACCCGGCATATGTAAAA-3'

58°C

ΔCS3

Δ647-675

OppA start

5'-GTGGCGGCCGCGCCTGCAGTTTTTTAG-3'

61°C

  

CS3 down

5'-GTACAGCTGTGGAGCATTTAAATATCT-3'

61°C

  

CS3 up

5'-GCTCCACAGCTGTACGATCCAAACTTCAA-3'

60°C

  

OppA end

5'-GGTCCATGGTGGGTACCAAAATAGACCCGGCATATGTAAAA-3

60°C

ΔWB

Δ712-727

OppA start

5'-GTGGCGGCCGCGCCTGCAGTTTTTTAG-3'

50°C

  

DC10 down

5'-ATATGCGTTGAAGTTTGGAT-3'

50°C

  

DC10 up

5'-TATAACGGTGTTGCTAGCACATAC-3'

58°C

  

OppA end

5'-GGGTCCATGGTGGGTACCAAAATAGACCCGGCATATGTAAAA-3'

58°C

WA3

874GKDSSGKS-GLQSYGKT881

OppA start

5'-GTGGCGGCCGCGCCTGCAGTTTTTTAG-3'

60°C

  

DC10 down

5'-TACAGATCTGTTGGTTCTATAGTTTTTCCATAACTCTGCAATCCAAAATC-3'

60°C

  

DC10 up

5'-CAACAGATCTGTATCAGTGGTCTGCAAT-3'

60°C

  

OppA end

5'-GGGTCCATGGTGGGTACCAAAATAGACCCGGCATATGTAAAA-3'

60°C