Figure 1

OppA variants. A. Schematical overview of proposed functional OppA regions and secondary structure predictions of the OppA variants. The binding sites of mAb BG11 and mAb DC10 are depicted with antibody icons. CS1, a conserved region of bacterial OppA proteins, is shown in diagonal strips, and conserved regions of mycoplasmal OppA proteins are depicted by dotted areas (CS2) and vertical strips (CS3). The ATP-binding site consists of the C-terminal localized Walker A (grid) and Walker B (horizontal strips) motifs. The deletion mutants were sign with gaps between the OppA bulks. Modified regions of the Walker A mutants were described below the OppA bulks. B. SDS-PAGE of the recombinant OppA mutants and wild type proteins P50, P60/P80, OppA^wt and the dephosphorylated OppA^ΔPi variant. The purified proteins were separated on a 9.5% SDS gel followed by Coomassie staining and the wild type OppA variants in addition by ProQ- staining demonstrating phosphorylations. SeeBlue Plus 2 Pre-Stained Standard from Invitrogen was used as molecular weight marker.