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Table 1 Composition of chemically defined media (CDM)

From: Expression of urease by Haemophilus influenzaeduring human respiratory tract infection and role in survival in an acid environment

Reagent Concentration
NaCl 0.1 M
K2SO4 5.75 mM
Na2EDTA 4 mM
NH4Cl 4 mM
K2HPO4 2 mM
KH2PO4 2 mM
Thiamine HCl 6 μM
Thiamine pyrophosphate 1 μM
Pantothenic acid 8 μM
d-Biotin 12 μM
Glucose 0.5%
Hypoxanthine 0.375 mM
Uracil 0 .45 mM
L-aspartic acid 3.75 mM
L-glutamic acid HCl 7.5 mM
L-arginine 0.875 mM
Glycine HCl 0.225 mM
L-serine 0.475 mM
L-leucine 0.7 mM
L-isoleucine 0.225 mM
L-valine 0.525 mM
L-tyrosine 0.4 mM
L-cysteine HCl 0.35 mM
L-cystine 0.15 mM
L-proline 0.45 mM
L-tryptophan 0.4 mM
L-threonine 0.425 mM
L-phenylalanine 0.15 mM
L-asparagine 0.2 mM
L-glutamine 0.35 mM
L-histidine HCl 0.125 mM
L-methionine 0.1 mM
L-alanine 1.125 mM
L-lysine 0.35 mM
Glutathione reduced 0.15 mM
HEPES 42 mM
NaHCO3 0.125 mM
Na acetate trihydrate 6.25 mM
Choline chloride salt 0.05 mM
Myo-inositol 1 μM
MgCl2 2.5 mM
CaCl2 0.6 mM
Fe(NO3)3 0.1 mM
Nicotinamide adenine dinucleotide 0.02 mM
Protoporphyrin IX 0.02 mM
Histidine 6 μM
Triethanolamine 0 .01%