Figure 8

Infection with the galU mutant of FT LVS elicits protective immunity WT FT LVS. C57Bl/6J mice (n = 5) that had survived intranasal challenge with the galU mutant FT strain and naïve control mice (n = 5) were challenged intranasally with 5 × 10⁴ CFU (50 × LD₅₀) of WT FT LVS eight weeks following the initial infection. The body weight (Panel A) and survival (Panel B) of mice were monitored for survival for 30 days. Statistical analyses of changes in body weight were performed via two-way ANOVA using a Bonferroni multiple comparisons post-test and p-values are indicated as follows: * P < 0.05 and *** P < 0.001. Statistical analysis of the survival data was performed using a Gehan-Breslow-Wilcoxon test (** indicates a p-value of 0.0043).