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Table 2 Number (percentage) of samples containing total bifidobacteria and B. pseudolongum in St-Marcellin and Brie processes
| Process/Methods | Production steps | ||||
|---|---|---|---|---|---|
| St-Marcellin |
Total n = 176 |
A n = 44 |
B n = 44 |
C n = 44 |
D n = 44 |
| Total bifidobacteria | |||||
| PCR 16S rDNA | 153 (87%) | 42 (95%) | 37 (84%) | 35 (80%) | 39 (89%) |
| PCR hsp60 gene | 154 (88%) | 42 (95%) | 38 (86%) | 35 (80%) | 39 (89%) |
| B. pseudolongum | |||||
| PCR RFLP (16S rDNA) | 135 (77%)/ | 41 (93%)/ | 28 (66%)/ | 34 (77%)/ | 32 (73%)/ |
| Real time PCR (hsp60 gene) | 120 (68%) | 35 (80%) | 27 (61%) | 27 (61%) | 31 (70%) |
| Brie |
Total
n = 120 |
A'
(n = 30) |
B'
(n = 30) |
C'
(n = 30) |
D'
(n = 30) |
| Total bifidobacteria | |||||
| PCR 16S rDNA | 107 (89%) | 29 (97%) | 21 (70%) | 28 (93%) | 29 (97%) |
| PCR hsp60 gene | 105 (88%) | 29 (97%) | 22 (73%) | 27 (90%) | 27 (90%) |
| B. pseudolongum | |||||
| PCR RFLP (16S rDNA) | 107 (89%) | 29 (97%) | 21 (70%) | 28 (93%) | 29 (97%) |
| Real time PCR (hsp60 gene) | ND | ND | ND | ND | ND |
