Identification of new IS711 insertion sites in Brucella abortus field isolates

BMC Microbiology

Table 1 Brucella strains used

		Genetic profile by:
Strain	Relevant features	RFLP IS711 AvaI-ClaI^a	AMOS enhanced PCR ^b	Reference
B. abortus 544	Reference strain of biovar 1	A	A	[24]
B. abortus 2308	USDA challenge strain; biovar 1	B	B	[25]
B. abortus RB51	Vaccine rough derivative from 2308	C	B	[26]
B. abortus B51^c	Biovar 1; milk isolate (Río Bueno, Chile; 2004)	A	A	This work
B. abortus B12^c	Biovar 1; milk isolate (Río Bueno, Chile; 2004)	D	A	[10]
B. abortus B16^d	Biovar 1; aborted fetus isolate (Osorno, Chile; 2002)	E	A	[10]
B. abortus B49^d	Biovar 1; aborted fetus isolate (Osorno, Chile; 2000)	E	A	This work
B. abortus B50^d	Biovar 1; aborted fetus isolate (Osorno, Chile; 2004)	E	A	This work
B. ovis 23/290	B. ovis reference strain	F	C	[24]
B. ceti NCTC 12891^T	B. ceti type strain	Np^e	Np	[27]
B. pinnipedialis NCTC 12890^T	B. pinnipedialis type strain	Np	Np	[27]
B. abortus 2308 Nal^R	Nalidixic acid resistant derivative of 2308 strain	Np	Np	[21]

^a IS profiles are shown in Figure 1.
^b A, B. abortus typical pattern; B, B. abortus 2308 pattern; C, B. ovis typical pattern.
^c B12 and B51 were isolated from the same sample.
^d B16, B49 and B50 are strains isolated from different outbreaks in the same flock.
^e Np: Not performed

ISSN: 1471-2180