Figure 3

Schematic representation of new IS 711 loci found in B. abortus field isolates. B12 (upper panel) and B16 and its related isolates (lower panel). The full-length 842 bp IS711 elements and their overlapping ORFs appear in grey. The Bru-RS1 element is shown as hatched box. The duplicated TA at the consensus YTAR site is shown below. Small black arrows represent the positions of site-specific primers. Numbers between primers indicate the molecular size of PCR products. The coordinates are based on the B. abortus 9-941 annotation. ORFs BruAb1_0734, BruAb1_0735 and BruAb1_0736 encode hypothetical proteins; lldP, L-lactate permease (BruAb1_0737); BruAb2_462 encodes a putative D-amino acid oxidase family protein; asnC, transcriptional regulator AsnC family (BruAb2_0459).