Factors influencing lysis time stochasticity in bacteriophage λ

BMC Microbiology

Table 2 Effect of late promoter activity, lysogen growth rate and KCN addition on the stochasticity of lysis time.

Treatment	n ^c	MLT (min)	SD (min)
p_R' activity
IN56 (1)^a	230	65.1	3.24
SYP026 (2)^a	128	61.9	3.20
SYP027 (3)^a	45	62.1	2.91
SYP043 (4)^a	43	74.3	9.22
SYP028 (5)^a	70	110.6	17.83
Growth rate
100% LB^b	230	65.1	3.24
20% LB	233	59.5	3.86
DM+Glc^b	125	70.3	6.30
DM+Gly^b	78	83.8	9.16
KCN addition
at 25 min	72	52.1	7.12
at 30 min	67	56.6	6.85
at 32 min	61	54.0	4.74
at 34 min	46	55.7	4.33
at 35 min	161	45.4	1.86
at 45 min	151	50.1	1.83
at 55 min	158	57.6	1.45

^aNumbers in the brackets indicate p_R' activity ranking with 1 being the highest and 5 being the lowest [50]; IN56 data is from Table 2.
^b100%LB data is from Table 2, strain IN56; DM, Davis minimal salts medium; Glc, glucose; Gly, glycerol.
C In some cases, the sample size n is the pooled number of cells observed across several days. Detailed information can be found in Table S2 of the addition file 1.

ISSN: 1471-2180