A multi locus variable number of tandem repeat analysis (MLVA) scheme for Streptococcus agalactiae genotyping

BMC Microbiology

Table 2 Primers used in the MLVA scheme

Forward primers	Sequence (5'-3')	Coordinates¹	Ref strains²	Reverse primers	Sequence (5'-3')	Coordinates¹
SAG2F	TCTTCCAAGTGGTGTCAACG	76270 - 76289	A909	SAG2R	CAACGTTTGGAGTTGCTTCA	76494 - 76513
SAG3F	CAAAAACGTGCTGCCTATGA	107351 - 107370	A909	SAG3R	CATCCCTCCTCCACCAAAA	107458 - 107476
SAG4F	GGTCAGTTTTTATTTATCGTAAGC	152991 - 153014	A909	SAG4R	AGTCTTGCGAAGGCAGACAC	153085 - 153104
SAG7F	TGGTGTTGATAAAGTTGATGTTCC	745963 - 745986	A909	SAG7R	GCCATATGAACTGCGGAAAC	746228 - 746247
SAG7bisF	ACCTATGCTCCCAGTGGTTC	111555 - 111574	NEM316	SAG7bisR	TCACTTAAGCGCACTGCAAC	112036 - 112055
SAG21F	TGAAAGAAGTGGATTTTTCCCTA	1062584 - 1062606	A909	SAG21R	AAAATAGGTTTTAGAACTTGGAAATCA	1062675 - 1062701
SAG22F	TGTAACACTAGCTCCAATTTGTTTT	1745819 - 1745843	A909	SAG22R	TCGGTCTTGTCTCAGCAATG	1746727 - 1746746

¹, Nucleotide coordinates in the reference strains (A909, NEM316) chosen for primer design
², Reference strain chosen for primer design

ISSN: 1471-2180